A highly efficient murine model of experimental myopia.
A highly efficient murine model of experimental myopia.
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DOI：
10.1038/s41598-018-20272-w
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发表时间：
2018-02-01
影响因子：
4.6
通讯作者：
Tsubota K
中科院分区：
文献类型：
--
作者：
Jiang X;Kurihara T;Kunimi H;Miyauchi M;Ikeda SI;Mori K;Tsubota K;Torii H;Tsubota K
尽管近视在全球大流行，但近视发病的精确分子机制在很大程度上仍然未知。这部分是因为缺乏有效的小鼠近视模型，允许以低成本进行遗传操作。在这里，我们报告了一个高度实用的和可重复的透镜诱导的近视模型，通过专门设计的框架和透镜的小鼠。直到负30屈光度的镜片，显示小鼠中的透镜屈光度依赖性近视诱导。表型明显强于形觉剥夺性近视。我们提出了使用最新设备精确评估近视状态的方案，包括屈光度、角膜曲率和眼轴长度。我们还发现，近视小鼠的眼睛表现出视动反应检查视力下降。最后，我们利用该模型证实了1%阿托品的抗近视作用，显示了其在药物筛选中的潜力。该方法在小鼠中的强表型、稳定的评价和基因操作的潜力将加速近视的转化研究。
Despite the global pandemic of myopia, the precise molecular mechanism of the onset of myopia remains largely unknown. This is partially because of the lack of efficient murine myopic models that allow genetic manipulation at low cost. Here we report a highly practical and reproducible lens-induced myopia model by specially designed frames and lenses for mice. A lens power dependent myopic induction in mice was shown until minus 30 diopter lenses. The phenotype was significantly stronger than form-deprivation myopia. We presented the protocol for precise evaluations of the state of myopia, including refraction, corneal curvature and axial length using up-to-date devices. We also found that myopic mouse eyes showed decreased visual acuity on optokinetic response examination. Finally, we confirmed the anti-myopic effect of 1% atropine using this model, which showed its potential in drug screening. The strong phenotype, stable evaluation and the potential for gene manipulation utilizing the presented method in mice will accelerate the translational research of myopia.
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