Cloning and sequencing of the Saccharomyces cerevisiae gene LYP1 coding for a lysine‐specific permease
Cloning and sequencing of the Saccharomyces cerevisiae gene LYP1 coding for a lysine‐specific permease
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编码赖氨酸特异性通透酶的酿酒酵母基因 LYP1 的克隆和测序
DOI：
10.1002/yea.320090711
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发表时间：
1993
期刊：
影响因子：
2.6
通讯作者：
M. Chevallier
中科院分区：
文献类型：
--
作者：
H. Sychrová;M. Chevallier
通过在赖氨酸通透酶缺陷的受体酵母细胞中互补克隆酿酒酵母的 LYP1 基因，并测定其核苷酸序列。发现一个由 1833 个核苷酸组成的开放阅读框，编码由 611 个氨基酸组成的多肽，计算出的分子量为 68 118。对推导的蛋白质一级结构的分析揭示了 10 个跨膜区域和 3 个潜在的 N 糖基化位点。对 LYP1 蛋白推导序列的分析表明其与其他酵母氨基酸通透酶（特别是 CAN1）以及大肠杆菌的赖氨酸特异性通透酶具有同源性。通过携带 LYP1 基因的多拷贝质粒转化的菌株，其赖氨酸最大摄取速度比野生型增加了 20 倍，而通透酶与其底物的亲和力没有变化。
The LYP1 gene of Saccharomyces cerevisiae was cloned by complementation in lysine‐permease‐deficint recipient yeast cells, and its nucleotide sequence was determined. An open reading frame of 1833 nucleotides was found encoding a polypeptide of 611 amino acids, with a calculated molecular weight of 68 118. Analysis of the deduced primary structure of the protein revealed ten membrane‐spanning regions and three potential N‐glycosylation sites. Analysis of the deduced sequence of protein LYP1 indicates homology with other yeast amino‐acid permeases, in particular with CAN1, and also the lysine‐specific permease of Escherichia coli. The strain transformed by a multi‐copy plasmid harbouring the LYP1 gene, showed a 20‐fold increase in the maximum velocity of lysine uptake over that in the wild type, with no changes in the affinity of the permease for its substrate.
酿酒酵母的组氨酸通透酶基因 (HIP1)。
影响因子：
3.5
作者：
Tanaka,J;Fink,GR
通讯作者：
Fink,GR