The Golgi-localization of yeast Emp47p depends on its di-lysine motif but is not affected by the ret1-1 mutation in alpha-COP.
The Golgi-localization of yeast Emp47p depends on its di-lysine motif but is not affected by the ret1-1 mutation in alpha-COP.
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酵母EMP47P的高尔基体定位取决于其二赖氨酸基序，但不受α-COP中RET1-1突变的影响。
DOI：
10.1083/jcb.131.4.895
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发表时间：
1995-11
影响因子：
7.8
通讯作者：
RIEZMAN, H
中科院分区：
文献类型：
--
作者：
SCHRODER, S;SCHIMMOLLER, F;SINGERKRUGER, B;RIEZMAN, H
酿酒酵母EMP 47基因编码一种非必需的I型跨膜蛋白，其序列与由ERGIC-53和VIP 36定义的一类细胞内凝集素同源。Emp 47 p的12个氨基酸的COOH末端胞质尾区以序列KTKLL结束，这与基于二赖氨酸的ER定位信号的共识一致。尽管存在这个基序，Emp 47 p被证明是一个稳定状态的高尔基体蛋白。Emp 47 p的二赖氨酸基序在移植到质膜蛋白Ste 2 p上时具有功能，赋予ER定位。然而，Emp 47 p的高尔基体定位需要二赖氨酸基序，并且显示出与其他二赖氨酸基序相同的电荷非依赖性、位置依赖性特征。已显示α-COP是二赖氨酸标记的蛋白质的ER定位所需的。与这一发现相一致，在α- COP突变体ret 1 -1中，Ste 2 p-Emp 47 p杂合蛋白被错误定位于细胞表面.令人惊讶的是，Emp 47 p的高尔基体定位不受ret 1 -1突变的影响。为了研究Emp 47 p是否像其他二赖氨酸标记的蛋白质一样经历从高尔基体到ER的逆行转运，我们开发了一种测定法来测量sec 12突变体中阻断正向转运后的这一步骤。在这些条件下，逆行运输导致特定的重新分配Emp 47 p从高尔基体的ER。这种回收发生在含有α 1，3甘露糖修饰的寡糖的高尔基体亚室，这表明它起源于中间或更晚的高尔基体室。因此，Emp 47 p在高尔基体和ER之间循环，并且需要二赖氨酸基序用于其在高尔基体中的α-COP独立的稳态定位。
The Saccharomyces cerevisiae EMP47 gene encodes a nonessential type-I transmembrane protein with sequence homology to a class of intracellular lectins defined by ERGIC-53 and VIP36. The 12-amino acid COOH-terminal cytoplasmic tail of Emp47p ends in the sequence KTKLL, which conforms with the consensus for di-lysine-based ER-localization signals. Despite the presence of this motif, Emp47p was shown to be a Golgi protein at steady-state. The di-lysine motif of Emp47p was functional when transplanted onto Ste2p, a plasma membrane protein, conferring ER localization. Nevertheless, the di-lysine motif was required for Golgi-localization of Emp47p and showed the same charge- independent, position-dependent characteristics of other di-lysine motifs. Alpha-COP has been shown to be required for ER localization of di-lysine-tagged proteins. Consistent with this finding, the Ste2p- Emp47p hybrid protein was mislocalized to the cell surface in the alpha- COP mutant, ret1-1. Surprisingly, the Golgi-localization of Emp47p was unaffected by the ret1-1 mutation. To investigate whether Emp47p undergoes retrograde transport from the Golgi to the ER like other di- lysine-tagged proteins we developed an assay to measure this step after block of forward transport in a sec12 mutant. Under these conditions retrograde transport led to a specific redistribution of Emp47p from the Golgi to the ER. This recycling occurred from a Golgi subcompartment containing alpha 1,3 mannose-modified oligosaccharides suggesting that it originated from a medial-or later Golgi compartment. Thus Emp47p cycles between the Golgi apparatus and the ER and requires a di-lysine motif for its alpha-COP-independent, steady state localization in the Golgi.
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影响因子：
7.8
作者：
FRANZUSOFF, A;REDDING, K;SCHEKMAN, R
通讯作者：
SCHEKMAN, R
影响因子：
4.8
作者：
ARAR, C;CARPENTIER, V;ROCHE, AC
通讯作者：
ROCHE, AC
影响因子：
64.5
作者：
BALCH, WE;MCCAFFERY, JM;FARQUHAR, MG
通讯作者：
FARQUHAR, MG
影响因子：
2.6
作者：
CUNNINGHAM, KW;WICKNER, WT
通讯作者：
WICKNER, WT
信号介导的膜蛋白从高尔基体中的膜蛋白回收到酵母中的ER。
DOI：
10.1083/jcb.127.3.653
发表时间：
1994-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gaynor EC;te Heesen S;Graham TR;Aebi M;Emr SD
通讯作者：
Emr SD