The Effect and Mechanism of lncRNA NR2F1-As1/miR-493-5p/MAP3K2 Axis in the Progression of Gastric Cancer.
The Effect and Mechanism of lncRNA NR2F1-As1/miR-493-5p/MAP3K2 Axis in the Progression of Gastric Cancer.
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DOI：
10.1155/2021/3881932
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发表时间：
2021
影响因子：
--
通讯作者：
Luo L
中科院分区：
文献类型：
--
作者：
Liao X;Wen L;Luo L
LncRNA NR2F1-AS1在一些人类肿瘤中被发现是癌基因，如乳腺癌、非小细胞肺癌和食管鳞癌。然而，NR2F1-AS1是否参与了胃癌的进展仍不清楚。RT-qPCR检测NR2F1-AS1、MAP3K2和miR-493-5p在胃癌组织和细胞中的表达。Western blotting检测MAP3K2蛋白表达。用四甲基偶氮唑盐比色法和流式细胞仪检测胃癌细胞的增殖和凋亡情况。用Transwell法检测细胞在GC细胞中的迁移。NR2F1-AS1、MAP3K2和miR-493-5p之间的关系通过双荧光素酶报告基因检测得到证实。与对照组相比，NR2F1-AS1和MAP3K2在胃癌组织和细胞中的表达增加。NR2F1-AS1和MAP3K2基因敲除可显著抑制胃癌细胞的增殖和迁移，促进细胞凋亡。此外，NR2F1-AS1是miR-493-5p的海绵基因，MAP3K2是miR-493-5p的下游基因。此外，miR-493-5p可显著降低MAP3K2的表达，NR2F1-AS1可拮抗miR-493-5p对MAP3K2的抑制作用。因此，NR2F1-AS1通过海绵miR-493-5p上调MAP3K2的表达来调节GC细胞的生长。
LncRNA NR2F1-AS1 has been identified as an oncogene in some human tumors, such as breast cancer, nonsmall cell lung cancer, and esophageal squamous cell carcinoma. Nonetheless, whether NR2F1-AS1 is involved in the progression of gastric cancer (GC) remains unknown. The expression patterns of NR2F1-AS1, MAP3K2, and miR-493-5p in GC tissues and cells were detected by RT-qPCR. The protein expression of MAP3K2 was assessed by the Western blotting assay. The MTT assay and flow cytometry were performed to measure cell proliferation and cell apoptosis in GC cells. The transwell assay was adopted to assess cell migration in GC cells. The relationship between NR2F1-AS1, MAP3K2, and miR-493-5p was verified by a dual-luciferase reporter assay. The increased NR2F1-AS1 and MAP3K2 expressions were discovered in GC tissues and cells compared with control groups. Knockdown of NR2F1-AS1 and MAP3K2 dramatically suppressed cell proliferation and migration, while it enhanced cell apoptosis in GC cells. In addition, NR2F1-AS1 was found to be a sponge of miR-493-5p, and MAP3K2 was a downstream gene of miR-493-5p. Moreover, the expression of MAP3K2 was notably reduced by miR-493-5p, and NR2F1-AS1 counteracted the inhibition of miR-493-5p. Thus, NR2F1-AS1 was verified to regulate GC cell progression by sponging miR-493-5p to upregulate MAP3K2 expression.
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