A DNA small molecular probe with increasing K(+) concentration promoted selectivity.
A DNA small molecular probe with increasing K(+) concentration promoted selectivity.
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DOI：
10.1039/d0ra06274j
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发表时间：
2021-04-21
期刊：
影响因子：
3.9
通讯作者：
Gong HY
中科院分区：
文献类型：
--
作者：
Gong YP;Yang J;Fang JW;Li Q;Yu ZY;Guan A;Gong HY
DNA小分子探针技术被认为是一种很有前途的DNA相关疾病诊断方法。大多数相关报告是在特定盐度下进行的。本文中，4-亚氨基-3-（吡啶-2-基）-4H-喹嗪-1-甲腈（IPQC）经由简易程序以高产率（85%）生成。结果表明，IPQC在低K+浓度（小于20 mM）下可作为检测ssDNA、dsDNA和G4 DNA的通用探针。然而，IPQC通过UV-vis和荧光响应在增加[K+]（例如，150 mM）条件下。离子气氛效应对DNA探针的研究具有指导意义。这为靶DNA传感探针的设计、选择和优化提供了指导。DNA小分子探针技术被认为是一种很有前途的DNA相关疾病诊断方法。
DNA small molecular probe study was considered as a promising approach to achieve DNA related disease diagnosis. Most related reports were performed under specific salinity. Herein, 4-imino-3-(pyridin-2-yl)-4H-quinolizine-1-carbonitrile (IPQC) was generated via a facile procedure with high yield (85%). It is found that IPQC could act as a universal probe for most tested ssDNA, dsDNA and G4 DNA in low [K+] concentration (less than 20 mM). However, IPQC showed highly selective G4 DNA binding via UV-vis and fluorescence response in increasing [K+] (e.g., 150 mM) conditions. The ion atmosphere effects are instructive for DNA probe exploration. This provides guidance for the design, selection and optimization of the probes for target DNA sensing. DNA small molecular probe study was considered as a promising approach to achieve DNA related disease diagnosis.
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