High energy collisions on tandem time-of-flight mass spectrometers.
High energy collisions on tandem time-of-flight mass spectrometers.
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DOI：
10.1007/s13361-012-0518-4
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发表时间：
2013-05
影响因子：
3.2
通讯作者：
Cotter RJ
中科院分区：
文献类型：
--
作者：
Cotter RJ
在引入基质辅助激光解吸（MALDI）、电喷雾电离（ESI）、轨道仪和现在普遍用于蛋白质组学和代谢组学的任何其他工具之前很久，最高性能的质谱仪是扇形仪器，通过将静电能量分析仪（E）与高场磁体（B）相结合来提供高分辨率的质量测量。在其鼎盛时期，四扇区质谱仪（或EBEB）是皇冠上的宝石，提供最高性能的串联质谱法，使用单次高能碰撞诱导碎片。在四极杆和串联三重四极杆仪器也得到越来越多的使用和普及的时候，尽管如此，与低碰撞能量的对应物相比，这些部门仍有一些明显的优势。飞行时间质谱仪是高电压、高真空仪器，与扇区有很多共同之处，并激发了利用单次高能碰撞的串联仪器的发展。在这个回顾中，我们讲述了我们自己的旅程，以生产高性能的飞行时间和串联，描述的基本理论，问题和优势，这样的仪器。测试脉冲碰撞理论（ICT）的实验强调了与扇形质谱仪的相似性，这一概念最初是在扇形质谱仪上开发的。应用程序提供了更广泛的碎片化，侧链裂解和电荷远程碎片化的例子，也是高能扇形质谱仪的特征。此外，所谓的弯曲场反射器使得能够设计更简单的仪器，收集和聚焦所有离子，并可能为临床、组织成像和微生物表征提供未来技术。
Long before the introduction of matrix-assisted laser desorption (MALDI), electrospray ionization (ESI), Orbitraps and any of the other tools that are now used ubiquitously for proteomics and metabolomics, the highest performance mass spectrometers were sector instruments, providing high resolution mass measurements by combining an electrostatic energy analyzer (E) with a high field magnet (B). In its heyday, the four sector mass spectrometer (or EBEB) was the crown jewel, providing the highest performance tandem mass spectrometry using single, high energy collisions to induce fragmentation. During a time in which quadrupole and tandem triple quadrupole instruments were also enjoying increased usage and popularity, there were nonetheless some clear advantages for sectors over their low collision energy counterparts. Time-of-flight mass spectrometers are high voltage, high vacuum instruments that have much in common with sectors and have inspired the development of tandem instruments exploiting single high energy collisions. In this retrospective we recount our own journey to produce high performance time-of-flights and tandems, describing the basic theory, problems and the advantages for such instruments. An experiment testing impulse collision theory (ICT) underscores the similarities with sector mass spectrometers where this concept was first developed. Applications provide examples of more extensive fragmentation, side chain cleavages and charge-remote fragmentation, also characteristic of high energy sector mass spectrometers. Moreover, the so-called curved-field reflectron has enabled the design of instruments that are simpler, collect and focus all of the ions, and may provide the future technology for the clinic, for tissue imaging and the characterization of microorganisms.
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影响因子：
7.4
作者：
Cheng, CF;Gross, ML
通讯作者：
Gross, ML
DOI：
10.1039/c39810000325
发表时间：
1981-01-01
影响因子：
--
作者：
BARBER, M;BORDOLI, RS;TYLER, AN
通讯作者：
TYLER, AN
影响因子：
4.4
作者：
Basir, Y;Anderson, SL
通讯作者：
Anderson, SL
影响因子：
7.4
作者：
BROWN, RS;LENNON, JJ
通讯作者：
LENNON, JJ
DOI：
10.1002/oms.1210281025
发表时间：
1993-10-01
期刊：
ORGANIC MASS SPECTROMETRY
影响因子：
--
作者：
CORNISH, TJ;COTTER, RJ
通讯作者：
COTTER, RJ