The Structure Inventory of the Nuclear Pore Complex.
The Structure Inventory of the Nuclear Pore Complex.
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DOI：
10.1016/j.jmb.2016.03.015
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发表时间：
2016-05-22
影响因子：
5.6
通讯作者：
Schwartz TU
中科院分区：
文献类型：
--
作者：
Schwartz TU
核孔复合体是细胞核和细胞质之间穿过核膜进行分子交换的主要通道。由于它的绝对大小估计为50-112 MDa，并且它由大约500-1000个单独的蛋白质组成，因此对于结构生物学家来说，它是一个难以研究的对象。在这里，我回顾了NPC构建模块的高分辨率结构的广泛集成。随着尺寸和复杂性的增加，这些最新的大型结构和组件现在可以用作混合方法的基础，主要与低温电子显微镜分析相结合，生成NPC的第一个基于结构的组件模型。展望未来，这些结构对于NPC的详细分析至关重要，包括功能，进化和组装。
The nuclear pore complex is the principal gateway for molecular exchange between nucleus and cytoplasm across the nuclear envelope. Due to its sheer size of estimated 50–112 MDa and its complex buildup from about 500–1000 individual proteins, it is a difficult object to study for structural biologists. Here I review the extensive ensemble of high-resolution structures of the building blocks of the NPC. Concurrent with the increase in size and complexity, these latest, large structures and assemblies can now be used as the basis for hybrid approaches, primarily in combination with cryo-electron microscopic analysis, generating the first structure-based assembly models of the NPC. Going forward, the structures will be critically important for a detailed analysis of the NPC, including function, evolution, and assembly.
影响因子：
7.8
作者：
Scheer, U;Franke, W W
通讯作者：
Franke, W W