HPMA-oligolysine copolymers for gene delivery: optimization of peptide length and polymer molecular weight.
HPMA-oligolysine copolymers for gene delivery: optimization of peptide length and polymer molecular weight.
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DOI：
10.1016/j.jconrel.2011.07.009
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发表时间：
2011-10-30
期刊：
影响因子：
--
通讯作者：
Pun SH
中科院分区：
文献类型：
--
作者：
Johnson RN;Chu DS;Shi J;Schellinger JG;Carlson PM;Pun SH
聚阳离子是体内非病毒基因转移最常用的材料之一。一些研究已经证明了聚合物结构和递送活性之间的敏感关系。在这项工作中，我们使用反向加成-断裂链转移（RAFT）聚合，以建立一个面板的N-（2-羟丙基（甲基丙烯酰胺））（HPMA）-低聚赖氨酸共聚物具有不同的肽长度和聚合物分子量。针对最佳DNA结合、在盐中的胶体稳定性、高转染效率和低细胞毒性来筛选该板。PBS中多聚物稳定性的增加与聚合物分子量的增加和肽长度的减少相关。含有K5和K10寡阳离子的共聚物以比K15的共聚物显著更高的效率转染培养的细胞。鉴定了四种符合所需标准的HPMA-低聚赖氨酸共聚物。用这些共聚物形成的聚合复合物在培养的细胞中表现出与聚（乙烯亚胺）PEI相当的盐稳定性和转染效率。
Polycations are one of the most frequently used classes of materials for non-viral gene transfer in vivo. Several studies have demonstrated a sensitive relationship between polymer structure and delivery activity. In this work, we used reverse addition-fragmentation chain transfer (RAFT) polymerization to build a panel of N-(2-hydroxypropyl(methacrylamide) (HPMA)-oligolysine copolymers with varying peptide length and polymer molecular weight. The panel was screened for optimal DNA-binding, colloidal stability in salt, high transfection efficiency, and low cytotoxicity. Increasing polyplex stability in PBS correlated with increasing polymer molecular weight and decreasing peptide length. Copolymers containing K5 and K10 oligocations transfected cultured cells with significantly higher efficiencies than copolymers of K15. Four HPMA-oligolysine copolymers were identified that met desired criteria. Polyplexes formed with these copolymers demonstrated both salt stability and transfection efficiencies on-par with poly(ethylenimine) PEI in cultured cells.
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DOI：
10.1016/j.jconrel.2008.10.004
发表时间：
2009-02-10
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Convertine AJ;Benoit DS;Duvall CL;Hoffman AS;Stayton PS
通讯作者：
Stayton PS
影响因子：
6.2
作者：
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通讯作者：
Emrick, Todd
影响因子：
10.8
作者：
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通讯作者：
Pun, S. H.
影响因子：
10.8
作者：
Männistö, M;Vanderkerken, S;Urtti, A
通讯作者：
Urtti, A
影响因子：
4.6
作者：
Aliabadi, Hamidreza Montazeri;Landry, Breanne;Uludag, Hasan
通讯作者：
Uludag, Hasan