Complete primary structure of prostatropin, a prostate epithelial cell growth factor.
Complete primary structure of prostatropin, a prostate epithelial cell growth factor.
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前列腺素（一种前列腺上皮细胞生长因子）的完整一级结构。
DOI：
10.1021/bi00366a003
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
McKeehan,WL
中科院分区：
文献类型：
--
作者：
Crabb,JW;Armes,LG;Carr,SA;Johnson,CM;Roberts,GD;Bordoli,RS;McKeehan,WL
1986年7月21日收到的修订版Mandarin摘要：牛脑前列腺素是前列腺上皮细胞生长的有效和必需的有丝分裂原。前列腺素的主要形式在单个氨基末端封闭链中含有154个氨基酸残基，对应于17 400的分子量。前列腺素的150个羧基末端残基的氨基酸序列是通过Edman降解主要由赖氨酰和谷氨酰残基切割产生的重叠肽而得到的。通过快速原子轰击质谱法分析氨基末端十四肽，确定阻断基团为乙酰基部分，串联质谱法提供了前12个残基的序列。前列腺素残基15-154含有牛脑多肽的序列，最近被描述为酸性成纤维细胞生长因子和I类肝素结合生长因子。前列腺素的前25个残基的序列是乙酰-Ala-（Gly，Glu）-Glu-Thr-Thr-Thr-Phe-Thr-Ala-Leu-Thr-Glu-Lys-Phe-Asn-Leu-Pro-Leu-Gly-Asn-Tyr-Lys-Lys-Pro。还原和羧甲基化的前列腺素具有促有丝分裂活性，表明半胱氨酸残基30，61和97之间的二硫键在功能上不是必需的。这些结果通过严格的结构分析表明，以前仅被描述为间充质和神经外胚层细胞有丝分裂原的脑源性多肽也是一种上皮细胞生长因子，可能参与支持前列腺增生和腺癌。e来自雄激素响应性大鼠前列腺肿瘤和大鼠和人的雄激素依赖性正常前列腺的分离的上皮细胞的雄激素非依赖性增殖需要在神经组织中浓缩的多肽（促前列腺激素）1（McKeehan等人，1984; Chaproniere & McKeehan，1986）。最近通过硫酸铵分级分离、肝素-琼脂糖色谱法和反相高效液相色谱法（RP-HPLC）2从牛脑中纯化了两种分子形式的前列腺促激素至均一（Crabb等人，1986年）。一种形式具有约16000的分子量和未封闭的氨基末端，另一种形式具有约18000的分子量和封闭的氨基末端。这两份表格分发给
Revised Manuscript Received July 21, 1986 abstract: Bovine brain prostatropin is a potent and essential mitogen for prostate epithelial cell growth. The major form of prostatropin contains 154 amino acid residues in a single amino terminally blocked chain corresponding to a molecular weight of 17 400. The amino acid sequence of the 150 carboxy-terminal residues of prostatropin was derived by Edman degradation of overlapping peptides primarily generated by cleavage at lysyl and glutamyl residues. Analysis of the amino-terminal tetradecapeptide by fast atom bombardment mass spectrometry identified the blocking group as an acetyl moiety, and tandem mass spectrometry provided thé sequence of the first 12 residues. Prostatropin residues 15-154 contain the sequence of bovine brain polypeptides recently described as acidic fibroblast growth factor and class I heparin-binding growth factor. The sequence of the first 25 residues of prostatropin is acetyl-Ala-(Gly, Glu)-Glu-Thr-Thr-Thr-Phe-Thr-Ala-Leu-Thr-Glu-Lys-Phe-Asn-Leu-Pro-Leu-Gly-Asn-Tyr-Lys-Lys-Pro. Reduced and carboxymethylated prostatropin exhibits mitogenic activity, suggesting that disulfide bonds among cysteine residues 30, 61, and 97 are not functionally essential. These results demonstrate by rigorousstructural analysis that the brain-derived polypeptide previously described only as a mesenchymal and neuroectodermal cell mitogen is also an epithelial cell growth factor that may be involved in support of prostate hyperplasia and ade-nocarcinoma. e androgen-independent proliferation of isolated epithelial cells from androgen-responsive rat prostate tumors and an-drogen-dependent normal prostate of rat and human requires polypeptides (prostatropins) that are concentrated in neural tissue1 (McKeehan et al., 1984; Chaproniere & McKeehan, 1986). Two molecular forms of prostatropins were recently purified to homogeneity from bovine brain by ammonium sulfate fractionation, heparin-agarose chromatography, and reverse-phase high-performance liquid chromatography (RP-HPLC) 2 (Crabb et al., 1986). One form had a molecular weight of about 16000 and an unblocked amino terminus, and the other form had a molecular weight of about 18 000 and a blocked amino terminus. The two forms were distributed
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疏水蛋白及其片段的反相高效液相色谱。
影响因子：
2.9
作者：
Tarr,GE;Crabb,JW
通讯作者：
Crabb,JW
通过质谱法鉴定蛋白质中翻译后修饰的氨基酸。
影响因子：
--
作者：
S. Carr;K. Biemann
通讯作者：
K. Biemann
影响因子：
2.9
作者：
BROWNING, JL;MATTALIANO, RJ;SMART, JE
通讯作者：
SMART, JE
还原蛋白和 S-羧甲基化蛋白的制备和酶水解。
影响因子：
4.8
作者：
A. M. Crestfield;S. Moore;W. Stein
通讯作者：
W. Stein
牛脑中多种形式的前列腺素（前列腺上皮细胞生长因子）的表征。
DOI：
--
发表时间：
1986
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
J. Crabb;L. Armes;Charles M. Johnson;W. Mckeehan
通讯作者：
W. Mckeehan