The Fibrinogen-like Globe of Tenascin-C Mediates Its Interactions with Neurocan and Phosphacan/Protein-tyrosine Phosphatase-ζ/β*
The Fibrinogen-like Globe of Tenascin-C Mediates Its Interactions with Neurocan and Phosphacan/Protein-tyrosine Phosphatase-ζ/β*
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Tenascin-C 的纤维蛋白原样球介导其与 Neurocan 和 Phosphacan/蛋白酪氨酸磷酸酶-ζ/β* 的相互作用
DOI：
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发表时间：
1997
影响因子：
4.8
通讯作者：
R. U. Margolis
中科院分区：
文献类型：
--
作者：
P. Milev;D. Fischer;M. Häring;T. Schulthess;R. Margolis;R. Chiquet‐Ehrismann;R. U. Margolis
两种神经组织特异性硫酸软骨素蛋白聚糖，neurocan和phosphacan（蛋白酪氨酸磷酸酶-β的胞外结构域），是腱生蛋白C的高亲和力配体。使用在人纤维肉瘤细胞中表达为重组蛋白的部分生腱蛋白-C，我们已经通过直接放射性配体结合测定和抑制研究证明，除了缺乏纤维蛋白原样地球仪的那些之外，磷酸根聚糖结合在所有缺失变体中被保留，并且磷酸根聚糖以与天然或重组生腱蛋白-C几乎相同的亲和力（Kd = 12 nm）结合到该单一结构域。然而，神经聚糖的最大结合需要纤维蛋白原地球仪和一些相邻的纤连蛋白III型重复序列。磷酸蛋白聚糖和神经蛋白聚糖与完整的腱生蛋白-C的结合以及磷酸蛋白聚糖与纤维蛋白原地球仪的结合在钙的存在下显著增加。软骨素酶处理的蛋白聚糖不影响其结合到任何天然生腱蛋白-C或任何重组蛋白，表明这些相互作用是由蛋白聚糖的核心蛋白，而不是通过糖胺聚糖链介导的。这些结果也与旋转阴影电子显微照片一致，其显示磷酸根蛋白聚糖为一端由球状结构域终止的棒状物，该球状结构域在磷酸根蛋白聚糖和生腱蛋白-C的混合物中经常与纤维蛋白原地球仪并列。C6神经胶质瘤细胞粘附并在仅含有表皮生长因子样结构域或纤连蛋白III型重复序列和纤维蛋白原地球仪的腱生蛋白-C的缺失变体上扩散。在这两种情况下，细胞粘附被类似浓度的phosphacan抑制，表明纤维蛋白原地球仪对于这种作用不是必需的，这显然是由phosphacan对细胞的直接作用介导的，而不是由其与生腱蛋白C上的蛋白聚糖结合位点的相互作用介导的。
Two nervous tissue-specific chondroitin sulfate proteoglycans, neurocan and phosphacan (the extracellular domain of protein-tyrosine phosphatase-ζ/β), are high-affinity ligands of tenascin-C. Using portions of tenascin-C expressed as recombinant proteins in human fibrosarcoma cells, we have demonstrated both by direct radioligand binding assays and inhibition studies that phosphacan binding is retained in all deletion variants except those lacking the fibrinogen-like globe and that phosphacan binds to this single domain with nearly the same affinity (K d ∼12 nm) as to native or recombinant tenascin-C. However, maximum binding of neurocan requires both the fibrinogen globe and some of the adjacent fibronectin type III repeats. Binding of phosphacan and neurocan to intact tenascin-C, and of phosphacan to the fibrinogen globe, is significantly increased in the presence of calcium. Chondroitinase treatment of the proteoglycans did not affect their binding to either native tenascin-C or to any of the recombinant proteins, demonstrating that these interactions are mediated by the proteoglycan core proteins rather than through the glycosaminoglycan chains. These results are also consistent with rotary shadowing electron micrographs that show phosphacan as a rod terminated at one end by a globular domain that is frequently seen apposed to the fibrinogen globe in mixtures of phosphacan and tenascin-C. C6 glioma cells adhere to and spread on deletion variants of tenascin-C containing only the epidermal growth factor-like domains or the fibronectin type III repeats and the fibrinogen globe. In both cases cell adhesion was inhibited by similar concentrations of phosphacan, demonstrating that the fibrinogen globe is not necessary for this effect, which is apparently mediated by a direct action of phosphacan on the cells rather than by its interaction with the proteoglycan binding site on tenascin-C.
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脑硫酸软骨素蛋白多糖中唾液酸化和硫酸化O-糖苷甘露糖连接寡糖的结构研究。
DOI：
10.1042/bj2450229
发表时间：
1987
期刊：
The Biochemical journal
影响因子：
--
作者：
Krusius,T;Reinhold,VN;Margolis,RK;Margolis,RU
通讯作者：
Margolis,RU
DOI：
10.1016/s0021-9258(18)41808-x
发表时间：
1992-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Uwe Rauch;L. Karthikeyan;P. Maurel;R. U. Margolis;R. Margolis
通讯作者：
Uwe Rauch;L. Karthikeyan;P. Maurel;R. U. Margolis;R. Margolis
大鼠受体型蛋白酪氨酸磷酸酶-zeta/β的核苷酸序列和分子变异体。
DOI：
10.3109/10425179509030989
发表时间：
1995
期刊：
DNA sequence : the journal of DNA sequencing and mapping
影响因子：
--
作者：
Maurel,P;Meyer-Puttlitz,B;Flad,M;Margolis,RU;Margolis,RK
通讯作者：
Margolis,RK
DOI：
10.1016/s0021-9258(18)53809-6
发表时间：
1993-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
I. Aukhil;P. Joshi;Y. Yan;H. Erickson
通讯作者：
I. Aukhil;P. Joshi;Y. Yan;H. Erickson
DOI：
--
发表时间：
1986-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Krusius;J. Finne;R. Margolis;R. U. Margolis
通讯作者：
T. Krusius;J. Finne;R. Margolis;R. U. Margolis