Understanding photoreceptor outer segment phagocytosis: use and utility of RPE cells in culture.
Understanding photoreceptor outer segment phagocytosis: use and utility of RPE cells in culture.
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DOI：
10.1016/j.exer.2014.01.010
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发表时间：
2014-09
影响因子：
3.4
通讯作者：
Finnemann, Silvia C.
中科院分区：
文献类型：
--
作者：
Mazzoni, Francesca;Safa, Hussein;Finnemann, Silvia C.
RPE细胞是人体内最活跃的吞噬细胞。在眼睛中，RPE细胞始终面对视杆和视锥感光细胞外节，但每天仅一次有助于远端外节尖端的脱落和清除吞噬作用。原位RPE吞噬作用的分析已经成功地鉴定了RPE吞噬机制的关键参与者。吞噬过程包括三个不同的阶段，识别/结合，内化和消化，每个阶段分别由吞噬细胞调节。培养中RPE细胞吞噬作用的研究允许专门分析和操纵这些不同的阶段，以确定其分子机制。在这里，我们比较的相似性和差异的原代，永生化，干细胞衍生的RPE细胞培养的RPE细胞在原位吞噬功能方面。我们特别讨论了RPE细胞培养吞噬试验的潜在缺陷。最后，我们指出吞噬试验的发展，为未来的研究考虑。
RPE cells are the most actively phagocytic cells in the human body. In the eye, RPE cells face rod and cone photoreceptor outer segments at all times but contribute to shedding and clearance phagocytosis of distal outer segment tips only once a day. Analysis of RPE phagocytosis in situ has succeeded in identifying key players of the RPE phagocytic mechanism. Phagocytic processes comprise three distinct phases, recognition/binding, internalization, and digestion, each of which is regulated separately by phagocytes. Studies of phagocytosis by RPE cells in culture allow specifically analyzing and manipulating these distinct phases to identify their molecular mechanisms. Here, we compare similarities and differences of primary, immortalized, and stem cell-derived RPE cells in culture to RPE cells in situ with respect to phagocytic function. We discuss in particular potential pitfalls of RPE cell culture phagocytosis assays. Finally, we point out considerations for phagocytosis assay development for future studies.
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DOI：
10.1084/jem.194.9.1289
发表时间：
2001-11-05
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Finnemann SC;Silverstein RL
通讯作者：
Silverstein RL
影响因子：
56.9
作者：
BESHARSE, JC;HOLLYFIELD, JG;RAYBORN, ME
通讯作者：
RAYBORN, ME
DOI：
10.1073/pnas.222551899
发表时间：
2002-11-12
影响因子：
11.1
作者：
Crabb, JW;Miyagi, M;Hollyfield, JG
通讯作者：
Hollyfield, JG
影响因子：
3.7
作者：
Colella P;Sommella A;Marrocco E;Di Vicino U;Polishchuk E;Garcia Garrido M;Seeliger MW;Polishchuk R;Auricchio A
通讯作者：
Auricchio A
影响因子：
4.8
作者：
Damek-Poprawa, Monika;Diemer, Tanja;Boesze-Battaglia, Kathleen
通讯作者：
Boesze-Battaglia, Kathleen