Novel positive regulatory role for the SPL6 transcription factor in the N TIR-NB-LRR receptor-mediated plant innate immunity.
Novel positive regulatory role for the SPL6 transcription factor in the N TIR-NB-LRR receptor-mediated plant innate immunity.
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DOI：
10.1371/journal.ppat.1003235
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发表时间：
2013-03
期刊：
影响因子：
6.7
通讯作者：
Dinesh-Kumar SP
中科院分区：
文献类型：
--
作者：
Padmanabhan MS;Ma S;Burch-Smith TM;Czymmek K;Huijser P;Dinesh-Kumar SP
在识别病原体编码的效应物之后，植物TIR-NB-LRR免疫受体通过一个很大程度上未知的机制诱导防御信号。我们确定了SQUAMOSA启动子结合蛋白（SBP）域转录因子SPL6在激活防御转录组与核定位免疫受体相关后的一个新的和保守的作用。在主动免疫应答中，烟叶TIR-NB-LRR N免疫受体在不同的核室中与NbSPL6结合。NbSPL6对n介导的烟草花叶病毒抗性至关重要。同样，假定的拟南芥同源物AtSPL6是TIR-NB-LRR RPS4对携带avrRps4效应物的丁香假单胞菌介导的抗性所必需的。转录组分析表明，AtSPL6正调控防御基因的一个子集。因此，病原体激活的核定位TIR-NB-LRR如N可以通过SPL6调控防御基因，其机制类似于CIITA和NLRC5等哺乳动物免疫受体诱导MHC基因。病原菌感染在世界范围内造成重大的作物经济损失。为了抵御病原体，植物使用核苷酸结合域和富含亮氨酸重复序列（NB-LRR）类免疫受体。虽然我们对植物NB-LRR如何识别病原体有一些了解，但我们对NB-LRR在免疫反应中的空间分布和动态知之甚少。一些植物nb - lrs存在于细胞的核室中，表明它们可能直接控制防御基因的表达。对烟草花叶病毒（TMV）感染提供免疫的烟草N免疫受体存在于细胞核中，并与SQUAMOSA启动子结合蛋白样6 （SPL6）转录因子相关。只有当TMV效应p50存在于细胞中时，这种关联才会被检测到。这表明N仅在主动防御反应中与SPL6结合。防御TMV需要SPL6功能。来自拟南芥的SPL6在抵抗细菌病原体丁香假单胞菌中表达AvrRps4效应物，并积极调节防御基因的表达。这些发现确定了来自不同植物物种的SPL6转录因子在防御病原体中的一种新的保守功能。这是首次证明spll型转录因子在防御中的作用。
Following the recognition of pathogen-encoded effectors, plant TIR-NB-LRR immune receptors induce defense signaling by a largely unknown mechanism. We identify a novel and conserved role for the SQUAMOSA PROMOTER BINDING PROTEIN (SBP)-domain transcription factor SPL6 in enabling the activation of the defense transcriptome following its association with a nuclear-localized immune receptor. During an active immune response, the Nicotiana TIR-NB-LRR N immune receptor associates with NbSPL6 within distinct nuclear compartments. NbSPL6 is essential for the N-mediated resistance to Tobacco mosaic virus. Similarly, the presumed Arabidopsis ortholog AtSPL6 is required for the resistance mediated by the TIR-NB-LRR RPS4 against Pseudomonas syringae carrying the avrRps4 effector. Transcriptome analysis indicates that AtSPL6 positively regulates a subset of defense genes. A pathogen-activated nuclear-localized TIR-NB-LRR like N can therefore regulate defense genes through SPL6 in a mechanism analogous to the induction of MHC genes by mammalian immune receptors like CIITA and NLRC5. Pathogen infection causes significant economic loss of crops worldwide. To fend off pathogens, plants use the Nucleotide-Binding domain and Leucine Rich Repeat (NB-LRR) class of immune receptors. Although we have some insight into how plant NB-LRRs recognizes pathogens, we know little about NB-LRR spatial distribution and dynamics during the immune response. Some plant NB-LRRs are present in the nuclear compartment of the cell suggesting that they may directly control defense gene expression. The tobacco N immune receptor that provides immunity against Tobacco mosaic virus (TMV) infection is present in the nucleus and associates with the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 6 (SPL6) transcription factor. This association is detected only when the TMV effector, p50, is present in the cell. This suggests that N associates with SPL6 only during an active defense response. SPL6 function is required for defense against TMV. SPL6 from Arabidopsis functions in resistance against the bacterial pathogen Pseudomonas syringae expressing the AvrRps4 effector and positively modulates defense gene expression. These findings define a novel conserved function for SPL6 transcription factor from different plants species in defense against pathogens. This is the first evidence for the function of SPL-type transcription factors in defense.
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影响因子：
6.7
作者：
García AV;Blanvillain-Baufumé S;Huibers RP;Wiermer M;Li G;Gobbato E;Rietz S;Parker JE
通讯作者：
Parker JE
影响因子：
64.5
作者：
Caplan, Jeffrey L.;Mamillapalli, Padmavathi;Dinesh-Kumar, S. P.
通讯作者：
Dinesh-Kumar, S. P.
影响因子：
7.2
作者：
Erickson, FL;Holzberg, S;Baker, B
通讯作者：
Baker, B
影响因子：
5.3
作者：
Alpatov, R;Munguba, GC;Sugrue, SP
通讯作者：
Sugrue, SP
DOI：
10.1073/pnas.97.26.14789
发表时间：
2000-12-19
影响因子：
11.1
作者：
Dinesh-Kumar, SP;Tham, WH;Baker, BJ
通讯作者：
Baker, BJ