Araldite as an embedding medium for electron microscopy.
Araldite as an embedding medium for electron microscopy.
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阿拉尔德石作为电子显微镜的嵌入培养基。
DOI：
10.1083/jcb.4.2.191
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发表时间：
1958-03-25
期刊：
影响因子：
--
通讯作者：
GLAUERT RH
中科院分区：
文献类型：
--
作者：
GLAUERT AM;GLAUERT RH
环氧树脂是电子显微镜嵌入的合适介质，因为它们凝固均匀且几乎没有收缩。已经开发出一种可溶于乙醇的 Araldite 环氧树脂混合物，可产生薄切片所需硬度的块体。对传统混合物的关键修改是选择增塑树脂与脂肪族酸酐作为硬化剂。最终块的硬度可以通过加入额外的增塑剂来改变，并且凝固速率可以通过使用胺促进剂来控制。通过调整各种成分的比例，爱牢达混合物的性能可以有很大的变化。包埋生物标本的程序与甲基丙烯酸酯的程序类似，但建议使用更长的浸泡时间以确保更粘稠的环氧树脂完全渗透。已经报道了各种标本精细结构保存的改进，并且典型的电子显微照片说明了本文。
Epoxy resins are suitable media for embedding for electron microscopy, as they set uniformly with virtually no shrinkage. A mixture of araldite epoxy resins has been developed which is soluble in ethanol, and which yields a block of the required hardness for thin sectioning. The critical modifications to the conventional mixtures are the choice of a plasticized resin in conjunction with an aliphatic anhydride as the hardener. The hardness of the final block can be varied by incorporating additional plasticizer, and the rate of setting can be controlled by the use of an amine accelerator. The properties of the araldite mixture can be varied quite widely by adjusting the proportions of the various constituents. The procedure for embedding biological specimens is similar to that employed with methacrylates, although longer soaking times are recommended to ensure the complete penetration of the more viscous epoxy resin. An improvement in the preservation of the fine structure of a variety of specimens has already been reported, and a typical electron microgram illustrates the present paper.
影响因子：
64.8
作者：
GLAUERT, AM;ROGERS, GE;GLAUERT, RH
通讯作者：
GLAUERT, RH