An Axon-Pathfinding Mechanism Preserves Epithelial Tissue Integrity.
An Axon-Pathfinding Mechanism Preserves Epithelial Tissue Integrity.
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DOI：
10.1016/j.cub.2020.09.061
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发表时间：
2020-12-21
期刊：
影响因子：
--
通讯作者：
Bergstralh DT
中科院分区：
文献类型：
--
作者：
Cammarota C;Finegan TM;Wilson TJ;Yang S;Bergstralh DT
上皮组织形成器官的边界，在那里它们执行一系列功能，包括分泌、吸收和保护。这些组织通常由离散的细胞层组成，细胞层只有一个细胞厚。在检查的多个系统中，上皮细胞在分裂前向顶端方向聚集和移动，可能是对相邻细胞拥挤的反应。由于这种运动，子细胞可能会从组织层中产生。这些移位的细胞的重新整合支持组织生长并维持组织结构。两种保守的IgCAM（免疫球蛋白超家族细胞粘附分子），神经胶质细胞（Nrg）和成束蛋白2（Fas 2），参与细胞在果蝇滤泡上皮的重新整合。与脊椎动物直系同源物L1 CAM和NCAM 1/2一样，Nrg和Fas 2是主要在神经系统发育背景下研究的细胞粘附分子。与此相一致，我们确定了另一种神经IgCAM，Fasciclin 3（Fas 3），作为一种重新整合因子。Nrg，Fas 2和Fas 3是昆虫分隔连接的组成部分，功能相当于脊椎动物紧密连接，但增殖卵泡细胞没有成熟的分隔连接，我们发现分隔连接蛋白neurexin IV不参与重新整合。在这里，我们表明上皮重新整合的作用方式与IgCAM介导的轴突生长和寻路相同;它不仅依赖于细胞外粘附，还依赖于IgCAM和基于侧血影蛋白的膜骨架之间的机械偶联。我们的工作表明，重新整合是由一个独特的上皮粘附组件，是组成和功能等同于轴突之间的连接。从组织单层中突出出来的上皮细胞会重新整合到组织单层中。Cammarota，Finegan等人表明，果蝇滤泡上皮中的细胞重新整合是通过先前涉及轴突寻路的机制发生的。
Epithelial tissues form the boundaries of organs, where they perform a range of functions, including secretion, absorption, and protection. These tissues are commonly composed of discrete cell layers—sheets of cells that are one-cell thick. In multiple systems examined, epithelial cells round up and move in the apical direction before dividing, likely in response to neighbor-cell crowding. Because of this movement, daughter cells may be born displaced from the tissue layer. Reintegration of these displaced cells supports tissue growth and maintains tissue architecture. Two conserved IgCAMs (immunoglobulin superfamily cell adhesion molecules), neuroglian (Nrg) and fasciclin 2 (Fas2), participate in cell reintegration in the Drosophila follicular epithelium. Like their vertebrate orthologs L1CAM and NCAM1/2, respectively, Nrg and Fas2 are cell adhesion molecules primarily studied in the context of nervous system development. Consistent with this, we identify another neural IgCAM, Fasciclin 3 (Fas3), as a reintegration factor. Nrg, Fas2, and Fas3 are components of the insect septate junction, the functional equivalent of the vertebrate tight junction, but proliferating follicle cells do not have mature septate junctions, and we find that the septate junction protein neurexin IV does not participate in reintegration. Here, we show that epithelial reintegration works in the same way as IgCAM-mediated axon growth and pathfinding; it relies not only on extracellular adhesion but also mechanical coupling between IgCAMs and the lateral spectrin-based membrane skeleton. Our work indicates that reintegration is mediated by a distinct epithelial adhesion assembly that is compositionally and functionally equivalent to junctions made between axons. Epithelial cells born protruding from a tissue monolayer will reintegrate into it. Cammarota, Finegan, et al. show that cell reintegration in the Drosophila follicular epithelium occurs via a mechanism previously implicated in axon pathfinding.
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DOI：
10.1083/jcb.200704081
发表时间：
2007-08-27
期刊：
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影响因子：
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作者：
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影响因子：
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