Effect of Astaxanthin from Phaffia Rhodozyma on T cell Population and Responsiveness to Mitogen in Splenic Mononuclear Cells of Male Broiler Chicks
Effect of Astaxanthin from Phaffia Rhodozyma on T cell Population and Responsiveness to Mitogen in Splenic Mononuclear Cells of Male Broiler Chicks
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红法夫酶虾青素对雄性肉鸡脾单核细胞 T 细胞群和有丝分裂原反应性的影响
DOI：
10.2141/jpsa.43.41
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发表时间：
2006
影响因子：
1.5
通讯作者：
Kazuaki Takahashi
中科院分区：
文献类型：
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作者：
T. Takimoto;Kan Sato;Y. Akiba;Kazuaki Takahashi
在雄性肉鸡中测定了饲喂红法夫酶提供的虾青素 (Ax) 对 T 细胞群的影响以及脾单核细胞 (MNC) 对有丝分裂原、刀豆球蛋白 A (Con A) 或植物血凝素 (PHA) 的反应性。 1周龄的小鸡随意饲喂含有0、20或100ppm Axe的饮食7天。饲喂含有 Axe 的饮食会降低 CD3 阳性细胞的百分比，这是脾淋巴细胞中 T 细胞群的指标。饲喂含有20ppm Ax的饮食降低了作为辅助T细胞指标估计的CD4阳性细胞的百分比，但饲喂含有100ppm Ax的饮食没有观察到调节。 CD8 阳性细胞的百分比（细胞毒性 T 细胞的指标）不受含有 20 或 100ppm Ax 的饲料的影响。饲喂含有 20ppm Ax 的饮食可增强 Con A 刺激的 T 细胞增殖，但不受 100ppm Ax 的影响。饲喂含有 20 和 100ppm Axe 的饮食可增强脾细胞针对 PHA 的增殖。饲喂含有 Ax 的饮食对 Con A（估计为 T 细胞增殖促进因子）刺激的脾细胞中白细胞介素 (IL)-2 mRNA 表达没有影响。通过饲喂含有 20ppm Ax 的饮食，IL-2 样活性略有降低，但显着降低。 IFN-γmRNA 表达（估计为 Con A 刺激条件下的 T 细胞激活剂）在饲喂含有 20ppm Ax 的饮食的雏鸡中较高。这些结果表明，饲喂含有 Ax 的红法夫酵母后，脾 T 细胞的增殖和细胞因子表达与膳食 Ax 浓度或总 Ax 摄入量无关。饲喂 20ppm Ax 后 T 细胞增殖的增强可能是由于鸡脾细胞中 IFN-γ 的变化而不是 IL-2 产生的变化引起的。
Effect of feeding astaxanthin (Ax) provided as Phaffia rhodozyma on T cell population and responsiveness to mitogen, concanavalin A (Con A) or phytohemaggulutinin (PHA), of splenic mononuclear cells (MNC) was determined in male broiler chicks. Chicks at1 week of age, were fed ad libitum on diets containing 0, 20 or 100ppm Ax for 7 days. Feeding the diet containing Ax decreased percentage of CD3-positive cells, an indicator of the T cell population in splenic lymphocytes. Feeding the diet containing 20ppm Ax decreased percentage of CD4-positive cells estimated as the indicator of helper T cells, but no modulation was observed by feeding the diet containing100ppm Ax. Percentage of CD8-positive cells, an indicator of cytotoxic T cell, was not affected by feeding diets containing either 20 or 100ppm Ax. T cell proliferation stimulated with Con A was enhanced by feeding diet containing 20ppm Ax, but not affected by 100ppm Ax. Feeding diets containing 20 and 100ppm Ax enhanced the proliferation of splenocytes against PHA. Feeding diets containing Ax showed no effect on interleukin(IL)-2mRNA expression in splenocytes stimulated by Con A estimated as the T cell proliferation promoting factor. IL-2 like activity was slightly, but significantly, decreased by feeding diet containing 20ppm Ax. IFN-γmRNA expression, estimated as a T cell activator under condition of Con A stimulation, was higher in chicks fed the diet containing 20ppm Ax. These results suggest that proliferation and cytokine expressions of splenic T cells in response to feeding Phaffia rhodozyma containing Ax were independent of the dietary Ax concentration or total Ax intake. The enhancement of T cell proliferation by feeding 20ppm Ax might be caused by changes in IFN-γ rather than IL-2 production in the chick splenocytes.
DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
Chen-lo H. Chen-Chen-lo-H.-Chen-2109482134;L. Lanier Ager;G. Larry Gartland;M D Cooper
通讯作者：
Chen-lo H. Chen-Chen-lo-H.-Chen-2109482134;L. Lanier Ager;G. Larry Gartland;M D Cooper
DOI：
10.1093/jn/125.10.2483
发表时间：
1995-10
期刊：
The Journal of nutrition
影响因子：
--
作者：
H. Jyonouchi;S. Sun;Y. Tomita;M. Gross
通讯作者：
H. Jyonouchi;S. Sun;Y. Tomita;M. Gross