Towards rationally designed biomanufacturing of therapeutic extracellular vesicles: impact of the bioproduction microenvironment.
Towards rationally designed biomanufacturing of therapeutic extracellular vesicles: impact of the bioproduction microenvironment.
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DOI：
10.1016/j.biotechadv.2018.09.001
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发表时间：
2018-12
影响因子：
16
通讯作者：
Jay SM
中科院分区：
文献类型：
--
作者：
Patel DB;Santoro M;Born LJ;Fisher JP;Jay SM
细胞外囊泡（EV），包括外泌体、微泡等，已成为多种应用的潜在疗法。 EV 在治疗不愈合伤口、心肌梗塞、骨关节炎、创伤性脑损伤、脊髓损伤以及许多其他损伤和疾病方面的疗效的临床前报告证明了这种新兴治疗方式的多功能性。电动汽车也已被证明对人类有效，临床试验正在进行中以进一步探索其潜力。然而，为了使 EV 成为一类新的临床治疗方法，必须解决与翻译相关的问题。例如，最初为细胞生物制造开发的方法（例如中空纤维生物反应器培养）已适用于电动汽车生产，但对细胞培养微环境如何具体影响电动汽车的了解有限，限制了合理设计和优化电动汽车生产和效力的可能性。在这篇综述中，我们讨论了该问题的当前知识，并描绘了未来研究的潜在重点领域，以实现基于 EV 的疗法的转化和广泛应用。
Extracellular vesicles (EVs), including exosomes, microvesicles, and others, have emerged as potential therapeutics for a variety of applications. Pre-clinical reports of EV efficacy in treatment of non-healing wounds, myocardial infarction, osteoarthritis, traumatic brain injury, spinal cord injury, and many other injuries and diseases demonstrate the versatility of this nascent therapeutic modality. EVs have also been demonstrated to be effective in humans, and clinical trials are underway to further explore their potential. However, for EVs to become a new class of clinical therapeutics, issues related to translation must be addressed. For example, approaches originally developed for cell biomanufacturing, such as hollow fiber bioreactor culture, have been adapted for EV production, but limited knowledge of how the cell culture microenvironment specifically impacts EVs restricts the possibility for rational design and optimization of EV production and potency. In this review, we discuss current knowledge of this issue and delineate potential focus areas for future research towards enabling translation and widespread application of EV-based therapeutics.
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作者：
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