Some electrical and pharmacological properties of gap junctions between adult ventricular myocytes.

Some electrical and pharmacological properties of gap junctions between adult ventricular myocytes.
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成人心室肌细胞之间间隙连接的一些电学和药理学特性。

DOI:
10.1152/ajpcell.1985.249.5.c447
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发表时间:
1985
期刊:
The American journal of physiology
影响因子:
--
通讯作者:
Bennett,MV
Bennett,MV
中科院分区:
--
文献类型:
--
作者:
White,RL;Spray,DC;CamposdeCarvalho,AC;Wittenberg,BA;Bennett,MV

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使用Wittenberg和罗宾逊的技术(Cell Tissue Res.216:231-251,1981)从成年大鼠心脏分离心室肌细胞。这些细胞表现出形态,输入电阻,时间常数,和兴奋性预期的细胞在完整的心脏组织。这些细胞对电子耦合,和交界处的电导是不受跨接电位或超极化的两个细胞。细胞对短暂暴露于100%CO2平衡或含有0.1 mM辛醇的培养基中,迅速可逆地降低了连接电导。我们的结论是,心室肌细胞对之间的缝隙连接具有像在许多其他组织的交界处的生理特性。该制剂可用于评价药物对心脏连接通讯的作用。
Ventricular myocytes were isolated from adult rat hearts using the technique of Wittenberg and Robinson (Cell Tissue Res. 216: 231-251, 1981). These cells exhibited morphology, input resistance, time constant, and excitability expected for cells in intact cardiac tissue. Pairs of these cells were electronically coupled, and junctional conductance was unaffected by transjunctional potential or hyperpolarization of both cells. Brief exposure of cell pairs to medium equilibrated with 100% CO2 or containing 0.1 mM octanol quickly and reversibly decreased junctional conductance. We conclude that gap junctions between pairs of ventricular myocytes possess physiological properties like those of junctions in many other tissues. This preparation will be useful in evaluating drug action on junctional communication in heart.
DOI: --
发表时间: 1966
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影响因子: --
作者:
S. Weidmann;A. Hodgkin
通讯作者: A. Hodgkin
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