The influence of hydroxyapatite particles on osteoclast cell activities.
The influence of hydroxyapatite particles on osteoclast cell activities.
复制标题
羟基磷灰石颗粒对破骨细胞活性的影响。
DOI：
10.1002/(sici)1097-4636(19990615)45:4
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Hwa‐Chang Liu
中科院分区：
文献类型：
--
作者：
Jui;Feng;T. Hung;Y. Tsuang;Walter H. Chang;Hwa‐Chang Liu
全关节置换术后无菌性松动是骨科手术中的主要问题。据报道，材料磨损产生的微小颗粒是种植体失败的主要原因。因此，对植入物材料中可能存在的磨损颗粒进行调查，可能会延长关节置换术后的寿命。羟基磷灰石(HA)是一种具有良好生物相容性的优良生物材料，已被广泛研究和报道。在这项研究中，我们使用体外成骨细胞/破骨细胞模型来测试不同大小的HA颗粒的生物相容性。原代破骨细胞/成骨细胞与不同大小的HA颗粒(0.5~3.0微米、37~53微米、177~205微米、420~841微米)共培养3h、1d、3d、7d。通过细胞计数和培养上清液中转化生长因子(TGF-β1)、碱性磷酸酶(ALP)、肿瘤坏死因子(TNF-α)、前列腺素(PGE2)和乳酸脱氢酶(LDH)的分泌来评价细胞对HA颗粒的反应。结果表明，与小于53微米的HA颗粒共培养的成骨细胞/破骨细胞发生了最显著的变化。细胞数量显著减少，且成骨细胞数量的变化更为明显。血清中转化生长因子-β1浓度显著降低，前列腺素E_2和乳酸脱氢酶浓度显著升高，而血清中肿瘤坏死因子-α和碱性磷酸酶滴度无明显变化。由此可见，较大的HA颗粒可能与骨细胞有很好的相容性，而较小尺寸的HA颗粒既能激活破骨细胞，又能减少成骨细胞的细胞数量。在初次全髋关节置换术中使用羟基磷灰石所产生的额外费用的合理性应进一步评估。
Aseptic loosening after total joint arthroplasty is a major problem in orthopedic surgery. Small particles from material wear have been reported as the main cause of implant failure. For this reason, investigation into possible wear particles from the materials used in the implant may lead to longevity after arthroplasty. Hydroxyapatite (HA) has been extensively investigated and reported as an excellent biomaterial with excellent biocompatibility. In this study, we used an in vitro osteoblast/osteoclast model to test the biocompatibility of various-sized HA particles. Primary osteoclasts/osteoblasts were co-cultured with different-sized HA particles (0.5-3.0 microm, 37-53 microm, 177-205 microm, and 420-841 microm) for 3 h, 1 day, 3 days, and 7 days. Cellular responses to the HA particles were evaluated by changes in cell counts and the secretion of transforming growth factor (TGF-beta1), alkaline phosphatase (ALP), tumor necrosis factor (TNF-alpha), prostaglandin (PGE2), and lactate dehydrogenase (LDH) in the supernatant of the culture media. The results showed that osteoblasts/osteoclasts co-cultured with HA particles smaller than 53 microm undergo the most significant changes. Cellular counts significantly decreased, and the changes were more obvious in the osteoblast population. There also was a significant decrease in TGF-beta1 concentration and a significant increase in PGE2 and LDH concentration, but there were no changes in the TNF-alpha or ALP titer. It can be concluded that larger HA particles may be quite compatible with bone cells while smaller-sized HA particles can both activate the osteoclasts and decrease the cell population of the osteoblasts. Justification for the additional expense incurred with the use of hydroxyapatite in primary total hip arthroplasty should be further evaluated.
成骨细胞在多孔磷酸钙陶瓷上的生长：生物相容性研究的体外模型。
影响因子：
14
作者：
Cheung,HS;Haak,MH
通讯作者：
Haak,MH