Homogeneity of Antibody Responses in Tuberculosis Patients
Homogeneity of Antibody Responses in Tuberculosis Patients
复制标题
结核病患者抗体反应的同质性
作者：
K. Samanich;J. Belisle;S. Laal
ABSTRACT The goals of the present study were twofold: (i) to compare the repertoires of antigens in culture filtrates of in vitro-grown Mycobacterium tuberculosis that are recognized by antibodies from noncavitary and cavitary tuberculosis (TB) patients and (ii) to determine the extent of variation that exists between the antigen profiles recognized by individual TB patients. Lipoarabinomannan-free culture filtrate proteins of M. tuberculosis were fractionated by one-dimensional (1-D) and 2-D polyacrylamide gel electrophoresis, and the Western blots were probed with sera from non-human immunodeficiency virus (non-HIV)-infected cavitary and noncavitary TB patients and from HIV-infected, noncavitary TB patients. In contrast to earlier studies based on recombinant antigens of M. tuberculosis which suggested that antibody responses in TB patients were heterogeneous (K. Lyashchenko et al., 1998, Infect. Immun. 66:3936–3940, 1998), our studies with native culture filtrate proteins show that the antibody responses in TB patients show significant homogeneity in being directed against a well-defined subset of antigens. Thus, there is a well-defined subset of culture filtrate antigens that elicits antibodies during noncavitary and cavitary disease. In addition, another set of antigens is recognized primarily by cavitary TB patients. The mapping with individual patient sera presented here suggests that serodiagnostic tests based on the subset of antigens recognized during both noncavitary and cavitary TB will enhance the sensitivity of antibody detection in TB patients, especially in difficult-to-diagnose, smear-negative, noncavitary TB patients.
人类免疫缺陷病毒感染中临床前结核病的替代标志物：针对结核分枝杆菌 88 kDa 分泌抗原的抗体。
DOI：
10.1086/514015
发表时间：
1997
期刊：
The Journal of infectious diseases
影响因子：
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作者：
Laal,S;Samanich,KM;Sonnenberg,MG;Belisle,JT;O'Leary,J;Simberkoff,MS;Zolla-Pazner,S
通讯作者：
Zolla-Pazner,S