DNA compaction by the higher-order assembly of PRH/Hex homeodomain protein oligomers.
DNA compaction by the higher-order assembly of PRH/Hex homeodomain protein oligomers.
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DOI：
10.1093/nar/gkq659
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发表时间：
2010-11
影响因子：
14.9
通讯作者：
Gaston K
中科院分区：
文献类型：
--
作者：
Soufi A;Sawasdichai A;Shukla A;Noy P;Dafforn T;Smith C;Jayaraman PS;Gaston K
蛋白质的自组织对于核结构的建立和维持以及对基因表达的调控是必不可少的。我们以前已经证明，富含Pro的同源结构域蛋白(PRH/Hex)自组装形成寡聚复合体，以高亲和力和特异性与PRH结合位点阵列结合。我们还表明，许多PRH靶基因包含适当间隔的PRH位点阵列，使该蛋白能够结合并调节转录。在这里，我们使用分析超速离心法和电子显微镜来进一步表征PRH齐聚物。我们使用相同的技术来证明PRH寡聚体与长DNA片段结合后自结合形成高度有序的组装。电子显微镜和线性二色性分析表明，PRH寡聚体可以形成蛋白质-DNA纤维，并且在没有其他蛋白质的情况下，PRH能够使DNA紧密结合。最后，我们发现DNA紧凑不足以抑制细胞中PRH靶基因的表达。我们的结论是，DNA紧凑是大的PRH寡聚体与一系列结合位点结合的结果，并且PRH在功能和结构上与细菌和古菌的LRP/ASNC蛋白质家族有关，这是一组以前被认为没有真核对等蛋白质的蛋白质。
Protein self-organization is essential for the establishment and maintenance of nuclear architecture and for the regulation of gene expression. We have shown previously that the Proline-Rich Homeodomain protein (PRH/Hex) self-assembles to form oligomeric complexes that bind to arrays of PRH binding sites with high affinity and specificity. We have also shown that many PRH target genes contain suitably spaced arrays of PRH sites that allow this protein to bind and regulate transcription. Here, we use analytical ultracentrifugation and electron microscopy to further characterize PRH oligomers. We use the same techniques to show that PRH oligomers bound to long DNA fragments self-associate to form highly ordered assemblies. Electron microscopy and linear dichroism reveal that PRH oligomers can form protein–DNA fibres and that PRH is able to compact DNA in the absence of other proteins. Finally, we show that DNA compaction is not sufficient for the repression of PRH target genes in cells. We conclude that DNA compaction is a consequence of the binding of large PRH oligomers to arrays of binding sites and that PRH is functionally and structurally related to the Lrp/AsnC family of proteins from bacteria and archaea, a group of proteins formerly thought to be without eukaryotic equivalents.
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影响因子：
11.4
作者：
Katsani, KR;Hajibagheri, MAN;Verrijzer, CP
通讯作者：
Verrijzer, CP
影响因子：
7.7
作者：
Jennings, Barbara H.;Wainwright, S. Mark;Ish-Horowicz, David
通讯作者：
Ish-Horowicz, David
DOI：
10.1016/s1570-0232(02)00740-7
发表时间：
2003-03-25
影响因子：
3
作者：
Butcher, AJ;Gaston, K;Jayaraman, PS
通讯作者：
Jayaraman, PS
影响因子：
4.1
作者：
Bess, KL;Swingler, TE;Jayaraman, PS
通讯作者：
Jayaraman, PS
影响因子：
5.8
作者：
D'Elia, AV;Tell, G;Damante, G
通讯作者：
Damante, G