iPS cell sheets created by a novel magnetite tissue engineering method for reparative angiogenesis.
iPS cell sheets created by a novel magnetite tissue engineering method for reparative angiogenesis.
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DOI：
10.1038/srep01418
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发表时间：
2013
影响因子：
4.6
通讯作者：
Murohara, Toyoaki
中科院分区：
文献类型：
--
作者：
Kito, Tetsutaro;Shibata, Rei;Ishii, Masakazu;Suzuki, Hirohiko;Himeno, Tatsuhito;Kataoka, Yoshiyuki;Yamamura, Yumiko;Yamamoto, Takashi;Nishio, Naomi;Ito, Sachiko;Numaguchi, Yasushi;Tanigawa, Tohru;Yamashita, Jun K.;Ouchi, Noriyuki;Honda, Hiroyuki;Isobe, Kenichi;Murohara, Toyoaki
血管生成细胞疗法代表了一种治疗缺血性疾病的新策略，但一些患者表现出较差的反应。我们研究了一种新的磁铁矿组织工程技术(MAG-TE)创建的诱导多能干细胞(IPS)细胞片用于修复血管生成的治疗潜力。将小鼠iPS细胞来源的Flk-1+细胞与磁性纳米粒脂质体(MCL)共同孵育。将MCL标记的Flk-1+细胞与稀释的细胞外基质(ECM)前体混合，在反面放置磁铁。磁化后的Flk-1+细胞在磁场力作用下形成多层细胞膜。根据激光多普勒血流和毛细血管密度分析，植入Flk-1+细胞片加速了裸鼠缺血后肢相对于对侧肢体的血运重建。Flk-1+细胞片还可增加缺血组织中血管内皮生长因子和碱性成纤维细胞生长因子的表达。用这种新的MAG-TE方法建立的iPS细胞来源的Flk-1+细胞片代表了一种很有前途的治疗性血管生成的新模式。
Angiogenic cell therapy represents a novel strategy for ischemic diseases, but some patients show poor responses. We investigated the therapeutic potential of an induced pluripotent stem (iPS) cell sheet created by a novel magnetite tissue engineering technology (Mag-TE) for reparative angiogenesis. Mouse iPS cell-derived Flk-1+ cells were incubated with magnetic nanoparticle-containing liposomes (MCLs). MCL-labeled Flk-1+ cells were mixed with diluted extracellular matrix (ECM) precursor and a magnet was placed on the reverse side. Magnetized Flk-1+ cells formed multi-layered cell sheets according to magnetic force. Implantation of the Flk-1+ cell sheet accelerated revascularization of ischemic hindlimbs relative to the contralateral limbs in nude mice as measured by laser Doppler blood flow and capillary density analyses. The Flk-1+ cell sheet also increased the expressions of VEGF and bFGF in ischemic tissue. iPS cell-derived Flk-1+ cell sheets created by this novel Mag-TE method represent a promising new modality for therapeutic angiogenesis.
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DOI：
10.1161/atvbaha.111.231100
发表时间：
2011-10-01
影响因子：
8.7
作者：
Ishii, Masakazu;Shibata, Rei;Murohara, Toyoaki
通讯作者：
Murohara, Toyoaki
影响因子：
3.3
作者：
Kajiguchi, Masahiro;Kondo, Takahisa;Murohara, Toyoaki
通讯作者：
Murohara, Toyoaki
影响因子：
37.8
作者：
Higashi, Y;Kimura, M;Yoshizumi, M
通讯作者：
Yoshizumi, M
影响因子：
6.2
作者：
Miyagawa, S;Sawa, Y;Matsuda, H
通讯作者：
Matsuda, H
影响因子：
15.9
作者：
Murohara, T;Asahara, T;Isner, JM
通讯作者：
Isner, JM