Cell-autonomous sex differences in gene expression in chicken bone marrow-derived macrophages.
Cell-autonomous sex differences in gene expression in chicken bone marrow-derived macrophages.
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DOI：
10.4049/jimmunol.1401982
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发表时间：
2015-03-01
期刊：
影响因子：
--
通讯作者：
Hume DA
中科院分区：
文献类型：
--
作者：
Garcia-Morales C;Nandi S;Zhao D;Sauter KA;Vervelde L;McBride D;Sang HM;Clinton M;Hume DA
我们已经确定了在重组鸡M-CSF（CSF 1）中从雄性和雌性鸡的骨髓中生长的巨噬细胞中基因表达的差异。用或不用细菌LPS处理24小时对细胞进行分析。在雄性和雌性巨噬细胞中，长时间LPS刺激诱导了大约600个转录物。在Z染色体上编码的许多转录本在雄性中表达高出1.6倍，反映了在同配性别中缺乏剂量补偿。一组较小的W染色体特异性基因仅在女性中表达。哺乳动物中的LPS信号传导与1型IFN应答基因的诱导相关。出乎意料的是，由于IFN是在鸡的Z染色体上编码的，因此在相同条件下，未受刺激的雌性鸟类巨噬细胞表达的一组已知的IFN诱导基因的水平比雄性细胞高得多。为了证实这些差异是不是性腺激素的作用的后果，我们诱导性腺性逆转改变发育中的鸡的激素环境和分析培养的巨噬细胞从男性，女性，和女性的性别逆转胚胎。性腺性逆转并没有改变性连锁或干扰素反应基因的性二态表达。我们认为，雌鸟补偿剂量减少的诱导型干扰素与更高的基础设定点的干扰素反应基因。
We have identified differences in gene expression in macrophages grown from the bone marrow of male and female chickens in recombinant chicken M-CSF (CSF1). Cells were profiled with or without treatment with bacterial LPS for 24 h. Approximately 600 transcripts were induced by prolonged LPS stimulation to an equal extent in the male and female macrophages. Many transcripts encoded on the Z chromosome were expressed ∼1.6-fold higher in males, reflecting a lack of dosage compensation in the homogametic sex. A smaller set of W chromosome–specific genes was expressed only in females. LPS signaling in mammals is associated with induction of type 1 IFN–responsive genes. Unexpectedly, because IFNs are encoded on the Z chromosome of chickens, unstimulated macrophages from the female birds expressed a set of known IFN-inducible genes at much higher levels than male cells under the same conditions. To confirm that these differences were not the consequence of the actions of gonadal hormones, we induced gonadal sex reversal to alter the hormonal environment of the developing chick and analyzed macrophages cultured from male, female, and female sex-reversed embryos. Gonadal sex reversal did not alter the sexually dimorphic expression of either sex-linked or IFN-responsive genes. We suggest that female birds compensate for the reduced dose of inducible IFN with a higher basal set point of IFN-responsive genes.
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影响因子：
4.4
作者：
Kapetanovic R;Fairbairn L;Downing A;Beraldi D;Sester DP;Freeman TC;Tuggle CK;Archibald AL;Hume DA
通讯作者：
Hume DA
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
5.5
作者：
Raza S;Barnett MW;Barnett-Itzhaki Z;Amit I;Hume DA;Freeman TC
通讯作者：
Freeman TC
影响因子：
4.8
作者：
Piganis, Rebecca A. R.;De Weerd, Nicole A.;Hertzog, Paul J.
通讯作者：
Hertzog, Paul J.
影响因子：
4.4
作者：
Kapetanovic, Ronan;Fairbairn, Lynsey;Hume, David A.
通讯作者：
Hume, David A.