NK cells are intrinsically functional in pigs with Severe Combined Immunodeficiency (SCID) caused by spontaneous mutations in the Artemis gene.
NK cells are intrinsically functional in pigs with Severe Combined Immunodeficiency (SCID) caused by spontaneous mutations in the Artemis gene.
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DOI：
10.1016/j.vetimm.2016.04.008
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发表时间：
2016-07
影响因子：
1.8
通讯作者：
Tuggle CK
中科院分区：
文献类型：
--
作者：
Powell EJ;Cunnick JE;Knetter SM;Loving CL;Waide EH;Dekkers JC;Tuggle CK
We have identified Severe Combined Immunodeficiency (SCID) in a line of Yorkshire pigs at Iowa State University. These SCID pigs lack B-cells and T-cells, but possess Natural Killer (NK) cells. This SCID phenotype is caused by recessive mutations in the Artemis gene. Interestingly, two human tumor cell lines, PANC-1 and A375-SM, survived after injection into these SCID pigs, but, as we demonstrate here, these cells, as well as K562 tumor cells, can be lysed in vitro by NK cells from SCID and non-SCID pigs. NK cells from both SCID and non-SCID pigs required activation in vitro with either recombinant human IL-2 or the combination of recombinant porcine IL-12 and IL-18 to kill tumor targets. We also showed that SCID NK cells could be activated to produce perforin, and perforin production was greatly enhanced in NK cells from both SCID and non-SCID pigs after IL-2 cytokine treatment. While CD16+, CD172− NK cells constituted an average of only 4% in non-SCID pigs, NK cells averaged 27% of the peripheral blood mononuclear cell population in SCID pigs. We found no significant differences in killing activity per NK cell between SCID and non-SCID pigs. We conclude that survival of human cancer cells in these SCID pigs is not due to an intrinsic defect in NK cell killing ability.
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DOI：
10.1126/science.1198687
发表时间：
2011-01-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Vivier E;Raulet DH;Moretta A;Caligiuri MA;Zitvogel L;Lanier LL;Yokoyama WM;Ugolini S
通讯作者：
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影响因子：
4.4
作者：
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DOI：
10.1128/cdli.9.6.1248-1252.2002
发表时间：
2002-11-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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