Isoflurane inhibits nicotinic acetylcholine receptor-mediated 22Na+ influx and muscarinic receptor-evoked cyclic GMP production in cultured bovine adrenal medullary cells
Isoflurane inhibits nicotinic acetylcholine receptor-mediated 22Na+ influx and muscarinic receptor-evoked cyclic GMP production in cultured bovine adrenal medullary cells
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异氟烷抑制培养牛肾上腺髓质细胞中烟碱乙酰胆碱受体介导的 22Na 流入和毒蕈碱受体诱发的环 GMP 产生
DOI：
10.1007/bf00169287
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
F. Izumi
中科院分区：
文献类型：
--
作者：
K. Minami;N. Yanagihara;Y. Toyohira;M. Tsutsui;A. Shigematsu;A. Wada;F. Izumi
用培养的牛肾上腺髓质细胞研究了异氟醚对三种促分泌素（烟碱激动剂、藜芦碱和高浓度K+）诱导的22 Na+内流、45 Ca ~（2+）内流、儿茶酚胺分泌和环鸟苷酸（cGMP）生成的影响。(1)异氟烷（1-6%）以浓度依赖性方式抑制卡巴胆碱、尼古丁和二甲基-4-苯基哌嗪刺激的儿茶酚胺分泌。异氟烷抑制卡巴胆碱诱发的22 Na+内流和45 Ca 2+内流的浓度与抑制儿茶酚胺分泌的浓度相似。异氟烷对儿茶酚胺分泌的抑制作用不能通过增加卡巴胆碱的浓度来克服。(2)当异氟醚浓度增加到4- 6%时，即临床使用浓度（1-2%）的2-3倍时，异氟醚对藜芦碱诱导的~（22）Na ~+内流、~（45）Ca ~（2+）内流和儿茶酚胺分泌的抑制作用明显。(3)高K+诱发的~（45）Ca ~（2+）内流和儿茶酚胺分泌不受异氟烷（1-6%）的影响。(4)异氟醚（1-6%）减弱毒蕈碱引起的环GMP的产生，但不是由心房钠尿肽或硝普钠引起的。这些结果表明，异氟醚，在临床麻醉浓度，抑制烟碱乙酰胆碱受体介导的细胞反应，以及毒蕈碱受体介导的环GMP在肾上腺髓质细胞的生产。
The effects of isoflurane on 22Na+ influx, 45Ca2+ influx, catecholamine secretion and cyclic GMP production induced by three kinds of secretagogue (nicotinic agonists, veratridine and a high concentration of K+) have been investigated using cultured bovine adrenal medullary cells. (1) Isoflurane (1–6%) inhibited catecholamine secretion stimulated by carbachol, nicotine and dimethyl-4-phenylpiperazinium in a concentration-dependent manner. Isoflurane suppressed carbachol-evoked 22Na+ influx and 45Ca2+ influx at concentrations similar to those which suppressed catecholamine secretion. The inhibition of catecholamine secretion by isoflurane was not overcome by increasing the concentration of carbachol. (2) The inhibitory effects of isoflurane on veratridine-induced 22Na+ influx, 45Ca2+ influx and catecholamine secretion became evident when the concentration of isoflurane was raised to 4–6%, i.e. 2–3 fold higher than the concentrations (1–2%) employed clinically. (3) High K+-evoked 45Ca2+ influx and catecholamine secretion were not affected by isoflurane (1–6%). (4) Isoflurane (1–6%) attenuated the production of cyclic GMP caused by muscarine, but not that caused by atrial natriuretic peptide or by sodium nitroprusside.These results suggest that isoflurane, at clinical anesthetic concentrations, inhibits nicotinic acetylcholine receptor-mediated cell responses as well as muscarinic receptor-mediated cyclic GMP production in adrenal medullary cells.
挥发性麻醉剂对大鼠海马突触传递的影响。
影响因子：
8.8
作者：
Pearce,RA;Stringer,JL;Lothman,EW
通讯作者：
Lothman,EW
异氟烷对气道平滑肌直接影响的地形差异。
影响因子：
5.7
作者：
Mazzeo,AJ;Cheng,EY;Stadnicka,A;Bosnjak,ZJ;Coon,RL;Kampine,JP
通讯作者：
Kampine,JP