Role of TrkB expression in rat adrenal gland during acute immobilization stress.
Role of TrkB expression in rat adrenal gland during acute immobilization stress.
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DOI：
10.1111/jnc.12030
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发表时间：
2013-01
影响因子：
4.7
通讯作者：
Tsukinoki K
中科院分区：
文献类型：
--
作者：
Kondo Y;To M;Saruta J;Hayashi T;Sugiyama H;Tsukinoki K
酪氨酸受体激酶B（Trk B）是脑源性神经营养因子（BDNF）的一种受体，在制动应激过程中肾上腺髓质的表达显著升高。通过用重组BDNF刺激嗜铬细胞证实体外释放儿茶酚胺。我们调查的作用，TrkB和BDNF的本地化在肾上腺制动应力60分钟。血儿茶酚胺水平增加刺激后，TrkB表达在肾上腺髓质60分钟的应力，但是，血儿茶酚胺水平没有增加肾上腺切除大鼠。此外，在60分钟的应激过程中，在肾上腺髓质中检测到BDNF mRNA和蛋白的表达。同样，在大鼠接受交感神经阻滞与普萘洛尔，BDNF mRNA和蛋白在肾上腺髓质中检测到60分钟的压力。这些结果表明，肾上腺髓质中TrkB的信号转导引起儿茶酚胺释放。此外，下丘脑-垂体-肾上腺轴和肾上腺中BDNF的自分泌信号都能引起儿茶酚胺的释放。BDNF-TrkB相互作用可能在制动应激时肾上腺髓质的正反馈回路中发挥作用。
Expression of tyrosine receptor kinase B (TrkB), a receptor for brain-derived neurotrophic factor (BDNF), is markedly elevated in the adrenal medulla during immobilization stress. Catecholamine release was confirmed in vitro by stimulating chromaffin cells with recombinant BDNF. We investigated the role of TrkB and the localization of BDNF in the adrenal gland during immobilization stress for 60 min. Blood catecholamine levels increased after stimulation with TrkB expressed in the adrenal medulla during 60-min stress; however, blood catecholamine levels did not increase in adrenalectomized rats. Furthermore, expression of BDNF mRNA and protein was detected in the adrenal medulla during 60-min stress. Similarly, in rats undergoing sympathetic nerve block with propranolol, BDNF mRNA and protein were detected in the adrenal medulla during 60-min stress. These results suggest that signal transduction of TrkB in the adrenal medulla evokes catecholamine release. In addition, catecholamine release was evoked by both the hypothalamic–pituitary–adrenal axis and autocrine signaling by BDNF in the adrenal gland. BDNF–TrkB interaction may play a role in a positive feedback loop in the adrenal medulla during immobilization stress.
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DOI：
10.1016/s0169-328x(03)00003-2
发表时间：
2003-03-17
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Michalski, B;Fahnestock, M
通讯作者：
Fahnestock, M
影响因子：
10.6
作者：
Karege, F;Bondolfi, G;Bertschy, G
通讯作者：
Bertschy, G
影响因子：
6.1
作者：
Aliaga, E.;Silhol, M.;Tapia-Arancibia, L.
通讯作者：
Tapia-Arancibia, L.
影响因子：
16.2
作者：
Higo, Takayasu;Hamada, Kozo;Mikoshiba, Katsuhiko
通讯作者：
Mikoshiba, Katsuhiko
影响因子：
5.3
作者：
Qian, Ming D.;Zhang, Jie;Cho, Seongeun
通讯作者：
Cho, Seongeun