Identification and quantification of flavonoids and chromes in Baeckea frutescens by using HPLC coupled with diode-array detection and quadruple time-of-flight mass spectrometry
Identification and quantification of flavonoids and chromes in Baeckea frutescens by using HPLC coupled with diode-array detection and quadruple time-of-flight mass spectrometry
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使用 HPLC 结合二极管阵列检测和四重飞行时间质谱法对 Baeckea frutescens 中的类黄酮和铬进行鉴定和定量
DOI：
10.1080/14786419.2014.987144
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发表时间：
2015-05
影响因子：
2.2
通讯作者：
Qiang Wang
中科院分区：
文献类型：
--
作者：
Yan-Bing Zhang;Hong-Min Liu;Jie Yang;Qiang Wang
本文首次报道了利用高效液相色谱法（HPLC）结合二极管阵列检测（DAD）和四倍飞行时间质谱法（Q-TOF/MS）鉴定和定量枇杷主要生物活性成分的方法。根据其保留行为、紫外谱和质谱信息，共鉴定或初步鉴定了24种化合物。该方法具有良好的线性、灵敏度、精密度、稳定性、重复性和准确性，可用于同时测定不同采收期不同部位的五种黄酮类化合物和一种色素的含量，其动态含量反映了适宜的采收期。采用多生物活性标记建立的HPLC-DAD-Q-TOF/MS方法可有效地评价白僵菌原料及相关产品的质量。
This article marks the first report on high-performance liquid chromatography (HPLC) coupled with diode-array detection (DAD) and quadruple time-of-flight mass spectrometry (Q-TOF/MS) for the identification and quantification of main bioactive constituents in Baeckea frutescens. In total, 24 compounds were identified or tentatively characterised based on their retention behaviours, UV profiles and MS fragment information. Furthermore, a validated method with good linearity, sensitivity, precision, stability, repeatability and accuracy was successfully applied for simultaneous determination of five flavonoids and one chromone in different plant parts of B. frutescens collected at different harvest times, and their dynamic contents revealed the appropriate harvest times. The established HPLC-DAD-Q-TOF/MS using multi-bioactive markers was proved to be a validated strategy for the quality evaluation on both raw materials and related products of B. frutescens.
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影响因子：
2
作者：
Bei-xi Jia;Yu-xin Zhou;Xiaoqing Chen;Xiao-Bing Wang;Jie Yang;M. Lai;Qiang Wang
通讯作者：
Bei-xi Jia;Yu-xin Zhou;Xiaoqing Chen;Xiao-Bing Wang;Jie Yang;M. Lai;Qiang Wang
影响因子：
6.1
作者：
Shi-Biao Wu;Jing Wu;Z. Yin;Jinzhu Zhang;C. Long;E. Kennelly;Shengping Zheng
通讯作者：
Shi-Biao Wu;Jing Wu;Z. Yin;Jinzhu Zhang;C. Long;E. Kennelly;Shengping Zheng
影响因子：
2.1
作者：
W. Tsui;Geoffrey D. Brown
通讯作者：
W. Tsui;Geoffrey D. Brown
DOI：
--
发表时间：
2008-10
期刊：
Yao xue xue bao = Acta pharmaceutica Sinica
影响因子：
--
作者：
Wen-jie Lu;Qi Ya;Jia-yuan Chen;Burning Liu
通讯作者：
Wen-jie Lu;Qi Ya;Jia-yuan Chen;Burning Liu
DOI：
--
发表时间：
2008
期刊：
Natural Product Research and Development
影响因子：
--
作者：
Liu Bu-ming
通讯作者：
Liu Bu-ming