Differential expression of chemokine receptors on peripheral blood B cells from patients with rheumatoid arthritis and systemic lupus erythematosus.
Differential expression of chemokine receptors on peripheral blood B cells from patients with rheumatoid arthritis and systemic lupus erythematosus.
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DOI：
10.1186/ar1776
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发表时间：
2005
影响因子：
4.9
通讯作者：
Berek C
中科院分区：
文献类型：
--
作者：
Henneken M;Dörner T;Burmester GR;Berek C
趋化因子及其受体在淋巴细胞的募集和定位中是必不可少的。为了解决B细胞迁移到类风湿性关节炎（RA）患者的发炎滑膜组织中的问题，分析外周血幼稚B细胞、记忆B细胞和浆细胞的趋化因子受体CXCR 3、CXCR 4、CXCR 5、CCR 5、CCR 6、CCR 7和CCR 9的细胞表面表达。为了比较，分析了患有自身免疫性疾病系统性红斑狼疮（SLE）或退行性疾病骨关节炎（OA）的患者的外周血中的B细胞。通过流式细胞术测量趋化因子受体的表达水平，并在不同患者组和健康个体之间进行比较。趋化因子受体表达分析显示，大多数外周血B细胞对CXCR 3、CXCR 4、CXCR 5、CCR 6和CCR 7呈阳性。而一小部分B细胞呈CCR5阳性，几乎没有CCR9的表达被发现。与健康人相比，在RA患者的一个显着的比例B细胞显示CXCR5和CCR6的表达降低和CXCR3的水平增加。CXCR5的下调与CXCR3的上调相关。在SLE患者中，CXCR5表达出现显著变化。通过分别用趋化因子CXCL 10和CXCL 12的迁移测定证明趋化因子受体CXCR 3和CXCR 4的功能性。我们的研究结果表明，慢性炎症导致外周血B细胞上的趋化因子受体表达的调节。然而，RA患者和SLE患者之间的差异指向受体表达的疾病特异性调节。这些差异可能影响B细胞的迁移行为。
Chemokines and their receptors are essential in the recruitment and positioning of lymphocytes. To address the question of B cell migration into the inflamed synovial tissue of patients with rheumatoid arthritis (RA), peripheral blood naive B cells, memory B cells and plasma cells were analyzed for cell surface expression of the chemokine receptors CXCR3, CXCR4, CXCR5, CCR5, CCR6, CCR7 and CCR9. For comparison, B cells in the peripheral blood of patients with the autoimmune disease systemic lupus erythematosus (SLE) or with the degenerative disease osteoarthritis (OA) were analyzed. Expression levels of chemokine receptors were measured by flow cytometry and were compared between the different patient groups and healthy individuals. The analysis of chemokine receptor expression showed that the majority of peripheral blood B cells is positive for CXCR3, CXCR4, CXCR5, CCR6 and CCR7. Whereas a small fraction of B cells were positive for CCR5, practically no expression of CCR9 was found. In comparison with healthy individuals, in patients with RA a significant fraction of B cells showed a decreased expression of CXCR5 and CCR6 and increased levels of CXCR3. The downregulation of CXCR5 correlated with an upregulation of CXCR3. In patients with SLE, significant changes in CXCR5 expression were seen. The functionality of the chemokine receptors CXCR3 and CXCR4 was demonstrated by transmigration assays with the chemokines CXCL10 and CXCL12, respectively. Our results suggest that chronic inflammation leads to modulation of chemokine receptor expression on peripheral blood B cells. However, differences between patients with RA and patients with SLE point toward a disease-specific regulation of receptor expression. These differences may influence the migrational behavior of B cells.
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影响因子：
5.5
作者：
Buckley, CD
通讯作者：
Buckley, CD
影响因子：
4.4
作者：
Hauser, AE;Debes, GF;Manz, RA
通讯作者：
Manz, RA
影响因子：
15.3
作者：
Bowman, E P;Campbell, J J;Soler, D;Dong, Z;Manlongat, N;Picarella, D;Hardy, R R;Butcher, E C
通讯作者：
Butcher, E C
影响因子：
--
作者：
Hutloff, A;B端chner, K;Kroczek, RA
通讯作者：
Kroczek, RA
影响因子：
20.3
作者：
Muehlinghaus, G;Cigliano, L;Manz, RA
通讯作者：
Manz, RA