DJ-1 Expression in Cervical Carcinoma and its Effects on Cell Viability and Apoptosis.
DJ-1 Expression in Cervical Carcinoma and its Effects on Cell Viability and Apoptosis.
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DOI：
10.12659/msm.896861
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发表时间：
2016-08-21
期刊：
影响因子：
--
通讯作者：
Gao W
中科院分区：
文献类型：
--
作者：
Wang H;Gao W
本研究旨在探讨DJ-1在宫颈癌组织中的表达及其对细胞活力和凋亡的影响。宫颈癌细胞系Hela和85个组织样本，包括45个原发肿瘤活检组织，30个癌旁组织和10个正常宫颈组织样本，用于本研究。采用免疫组化法检测DJ-1在宫颈癌组织、癌旁组织及正常宫颈组织中的表达。用RT-PCR和Western blot检测DJ-1在Hela细胞中的表达。干扰DJ-1并转染siRNA，MTT法检测细胞活力，流式细胞仪检测细胞凋亡。另外，检测了磷酸酶和张力蛋白同源物（PTEN）、AKT和磷酸化AKT（P-AKT）的表达。免疫组化结果显示DJ-1在宫颈癌组织中呈高表达。在Hela细胞中，DJ-1的表达明显高于正常对照组（P<0.05）。DJ-1 siRNA处理后，细胞存活率显著降低（P<0.05），凋亡细胞百分比显著增加（P<0.05）。DJ-1 siRNA治疗组PTEN和AKT的表达明显高于对照组（P<0.05）。DJ-1 siRNA治疗组p-AKT的表达明显低于对照组和DJ-1过表达组（P<0.05）。DJ-1表达的异常上调可能是宫颈癌发生发展的重要环节。
This study aimed to investigate the expression of DJ-1 in cervical carcinoma and its effects on cell viability and apoptosis. Cervical carcinoma cell line Hela and 85 tissue samples, including 45 primary tumor biopsies, 30 para-carcinoma tissues, and 10 normal cervical tissues samples were used in this study. The expressions of DJ-1 in cervical carcinoma tissue, para-carcinoma tissue, and normal tissue samples were investigated by immunohistochemistry. DJ-1 expression in Hela cells was also investigated by quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. DJ-1 was interfered and transfected with siRNA, then cell viability and apoptosis were assayed by MTT and flow cytometry, respectively. Additionally, the expressions of phosphatase and tensin homolog (PTEN), AKT, and phospho-AKT (P-AKT) were detected. Immunohistochemistry results showed that DJ-1 was highly expressed in cervical carcinoma tissues. In Hela cells, the expression of DJ-1 was significantly higher than that in normal controls (P<0.05). When cells were treated with DJ-1 siRNA, the cell viability decreased significantly (P<0.05), and the percentage of apoptosis cells increased significantly (P<0.05). In addition, the expressions of PTEN and AKT were significantly higher in the DJ-1 siRNA treatment group than those in the control group (P<0.05). The expression of p-AKT was significantly lower in the DJ-1 siRNA treatment group than in the control group and the DJ-1 over-expression group (P<0.05). The aberrant up-regulation of DJ-1 expression might be an important step in the pathogenesis of cervical carcinoma.
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作者：
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