Long-term maintenance of luteinizing hormone-responsive testosterone formation by primary rat Leydig cells in vitro
Long-term maintenance of luteinizing hormone-responsive testosterone formation by primary rat Leydig cells in vitro
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原代大鼠 Leydig 细胞在体外长期维持黄体生成素反应性睾酮形成
DOI：
10.1016/j.mce.2018.04.008
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发表时间：
2018-11
影响因子：
4.1
通讯作者：
Chen H.
中科院分区：
文献类型：
--
作者：
Wang Y;Huang S;Wang Z;Chen F;Chen P;Zhao X;Lin H;Ge R;Zirkin B;Chen H.
培养的原代Leydig细胞不能在体外维持促黄体生成激素（LH）反应性睾酮形成超过3-5天，这在测试调节因子或环境毒物的营养效应方面提出了一个主要挑战。我们的主要目标是建立培养条件足以维持LH反应性睾丸激素形成Leydig细胞至少一个月。当分离的大鼠成年Leydig细胞在含有胰岛素（10μg/ml）、PDGFAA（10 ng/ml）、脂蛋白（0.25 mg/ml）、马血清（1%）和次最大浓度LH（0.2 ng/ml）的DMEM/F12和M199培养基中培养时，细胞在体外保持产生睾酮的能力至少4周。通过使用该系统的长期培养条件，我们能够检测到低水平毒物MEHP（邻苯二甲酸单（2-乙基己基）酯）对睾酮产生的抑制作用，MEHP是增塑剂DEHP的活性代谢产物，短期培养未检测到。
The inability of cultured primary Leydig cells to maintain luteinizing hormone (LH)-responsive testosterone formation in vitro for more than 3–5 days has presented a major challenge in testing trophic effects of regulatory factors or environmental toxicants. Our primary objective was to establish culture conditions sufficient to maintain LH-responsive testosterone formation by Leydig cells for at least a month. When isolated rat adult Leydig cells were cultured in DMEM/F12 and M199 culture medium containing insulin (10μg/ml), PDGFAA (10 ng/ml), lipoprotein (0.25 mg/ml), horse serum (1%) and a submaximal concentration of LH (0.2 ng/ml), the cells retained the ability to produce testosterone in vitro for at least 4 weeks. By using the longer-term culture conditions of this system, we were able to detect suppressive effects on testosterone production by low levels of the toxicant MEHP (mono-(2-ethylhexyl) phthalate), an active metabolite of the plasticizer DEHP, that were not detected by short-term culture.
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影响因子：
3.6
作者：
M. Hedger;A. Leung;D. Robertson;D. D. de Kretser-D.;G. Risbridger
通讯作者：
M. Hedger;A. Leung;D. Robertson;D. D. de Kretser-D.;G. Risbridger
影响因子：
5.3
作者：
Ying Zhang;N. Fatima;M. Dufau
通讯作者：
Ying Zhang;N. Fatima;M. Dufau
DOI：
10.1080/10937404.2010.483176
发表时间：
2010-02
期刊：
Journal of toxicology and environmental health. Part B, Critical reviews
影响因子：
--
作者：
Krewski D;Acosta D Jr;Andersen M;Anderson H;Bailar JC 3rd;Boekelheide K;Brent R;Charnley G;Cheung VG;Green S Jr;Kelsey KT;Kerkvliet NI;Li AA;McCray L;Meyer O;Patterson RD;Pennie W;Scala RA;Solomon GM;Stephens M;Yager J;Zeise L
通讯作者：
Zeise L
DOI：
10.1007/bf02629089
发表时间：
1988-06-01
期刊：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY
影响因子：
--
作者：
KLINEFELTER, GR;EWING, LL
通讯作者：
EWING, LL
影响因子：
4
作者：
Missaghian, Elika;Kempna, Petra;Flueck, Christa E.
通讯作者：
Flueck, Christa E.