Comparing the cytotoxicity of electronic cigarette fluids, aerosols and solvents.
Comparing the cytotoxicity of electronic cigarette fluids, aerosols and solvents.
复制标题
比较电子香烟液，气溶胶和溶剂的细胞毒性。
DOI：
10.1136/tobaccocontrol-2016-053472
复制
发表时间：
2018-05
期刊：
影响因子：
5.2
通讯作者：
Talbot P
中科院分区：
文献类型：
--
作者：
Behar RZ;Wang Y;Talbot P
随着数以千计的电子烟补充液不断被配制和分销，越来越需要了解这些产品中使用的调味化学品和溶剂的细胞毒性，以确保它们的安全。本研究的目的是使用体外培养的细胞来比较电子烟填充液/溶剂及其相应气雾剂的细胞毒性。使用 MTT（3-（4,5-二甲基噻唑2-基）−2,5-二苯基溴化四唑）测定法筛选液体和气雾剂形式的电子烟填充液和自制产品的细胞毒性。比较了人肺成纤维细胞、肺上皮细胞（A549）和人胚胎干细胞对液体和气溶胶的敏感性。气雾剂是使用 Johnson Creek 的 Vea 雾化器式电子烟生产的。为雾化产品建立了效力等级。我们的数据显示，(1) 电子烟气雾剂可以在培养细胞中产生细胞毒性作用，(2) 在比较补充液及其相应的气雾剂时发现了四种细胞毒性模式，(3) 液体在 74% 的时间内准确预测了气雾剂细胞毒性，(4) 干细胞通常比分化细胞对气雾剂更敏感，(5) 由仅含甘油的补充液制成的气雾剂中有 91%即使在低电压下产生，也具有细胞毒性。我们的数据显示，各种口味/品牌的电子烟填充液及其气溶胶具有细胞毒性，并表明需要进一步评估电子烟产品，以更好地了解其潜在的健康影响。
As thousands of electronic cigarette (e-cigarette) refill fluids continue to be formulated and distributed, there is a growing need to understand the cytotoxicity of the flavouring chemicals and solvents used in these products to ensure they are safe. The purpose of this study was to compare the cytotoxicity of e-cigarette refill fluids/solvents and their corresponding aerosols using in vitro cultured cells. E-cigarette refill fluids and do-it-yourself products were screened in liquid and aerosol form for cytotoxicity using the MTT (3-(4,5-dimethylthiazol2-yl)−2,5-diphenyltetrazolium bromide) assay. The sensitivity of human pulmonary fibroblasts, lung epithelial cells (A549) and human embryonic stem cells to liquids and aerosols was compared. Aerosols were produced using Johnson Creek’s Vea cartomizer style e-cigarette. A hierarchy of potency was established for the aerosolised products. Our data show that (1) e-cigarette aerosols can produce cytotoxic effects in cultured cells, (2) four patterns of cytotoxicity were found when comparing refill fluids and their corresponding aerosols, (3) fluids accurately predicted aerosol cytotoxicity 74% of the time, (4) stem cells were often more sensitive to aerosols than differentiated cells and (5) 91% of the aerosols made from refill fluids containing only glycerin were cytotoxic, even when produced at a low voltage. Our data show that various flavours/brands of e-cigarette refill fluids and their aerosols are cytotoxic and demonstrate the need for further evaluation of e-cigarette products to better understand their potential health effects.
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DOI：
10.3390/ijerph111111192
发表时间：
2014-10-28
影响因子：
--
作者：
Bekki K;Uchiyama S;Ohta K;Inaba Y;Nakagome H;Kunugita N
通讯作者：
Kunugita N
影响因子：
--
作者：
Behar, Rachel Z;Bahl, Vasundhra;Talbot, Prue
通讯作者：
Talbot, Prue
影响因子：
6
作者：
Hubbs, Ann F.;Cumpston, Amy M.;Sriram, Krishnan
通讯作者：
Sriram, Krishnan
影响因子：
3.2
作者：
Behar, R. Z.;Davis, B.;Talbot, P.
通讯作者：
Talbot, P.
影响因子：
15.9
作者：
Kitamura, Hideya;Cambier, Stephanie;Nishimura, Stephen L.
通讯作者：
Nishimura, Stephen L.