Messenger RNA expression of heat shock proteins (HSPs) during ocular development.
Messenger RNA expression of heat shock proteins (HSPs) during ocular development.
复制标题
眼部发育过程中热休克蛋白 (HSP) 的信使 RNA 表达。
作者：
Yasuyuki Tanaka;K. Kobayashi;M. Kita;S. Kinoshita;J. Imanishi
热休克蛋白（HSPs）被认为是一种分子伴侣，在调节正常蛋白质折叠和防止蛋白质结构在各种环境胁迫条件下受到损伤方面发挥一定的作用。我们研究了主要HSP家族，HSP 60，70和90家族和小HSP 32，在mRNA水平上在眼发育中的表达。原位杂交检测HSP 32、HSP 60、HSP 70、HSP 84、HSP 86和热休克相关蛋白（HSC）70 mRNA的表达。HSC 70、HSP 84、HSP 86和HSP 60 mRNA在早期阶段3至5（对应于胚胎日龄（E）11.5至E14.5）的所有眼组织中强烈表达。在6 ~ 7期（E15.5 ~ E18.5），这4种mRNA在大部分眼组织中的表达明显减少，而在视网膜中仍检测到强烈的HSC 70和HSP 86 mRNA表达。HSP 32和HSP 70 mRNA在任何阶段均未检测到。这些结果表明，热休克蛋白的表达是通过眼器官发生的发育调控，这些蛋白可能在眼发育中发挥重要作用。
The heat shock proteins (HSPs) are believed to act as molecular chaperones which appear to play some roles in regulation of normal protein folding and also in preventing damage to protein structures under various conditions of environmental stress. We examined the expression of the major HSP families, HSP60, 70 and 90 families and small HSP32, at the mRNA level in ocular development. Expression of HSP32, HSP60, HSP70, HSP84, HSP86 and heat shock cognate protein (HSC)70 mRNAs was examined by in situ hybridization. HSC70, HSP84, HSP86 and HSP60 mRNAs were expressed strongly in all ocular tissues during early stages 3 to 5, corresponding to embryonic day (E)11.5 to E14.5. At stages 6 to 7 (E15.5 to E18.5), the expression of these four mRNA species was decreased markedly in most ocular tissue, while in the retina strong HSC70 and HSP86 mRNA expression was still detected. HSP32 and HSP70 mRNAs were not detected at any stage. These results suggest that the expression of HSPs is developmentally regulated through ocular organogenesis, and the proteins may play some important roles in ocular development.
影响因子：
3.5
作者：
HUNT, C;CALDERWOOD, S
通讯作者：
CALDERWOOD, S