Increased single strand breaks in DNA of lymphocytes from diabetic subjects.
Increased single strand breaks in DNA of lymphocytes from diabetic subjects.
复制标题
糖尿病受试者淋巴细胞 DNA 中的单链断裂增加。
DOI：
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复制
发表时间：
1987
影响因子：
15.9
通讯作者：
H. Wong
中科院分区：
文献类型：
--
作者：
M. Lorenzi;D. Montisano;S. Toledo;H. Wong
糖尿病慢性并发症的某些方面表明，随着时间的推移，异常代谢环境导致某些细胞群发生不可逆转的变化。由于我们之前观察到高葡萄糖浓度诱导培养的人内皮细胞DNA单链断裂增加，我们已经研究了来自体内糖尿病环境的细胞是否会发生同样的异常。从21名I型糖尿病患者和年龄和性别匹配的对照组中获得的外周血淋巴细胞在碱中解绕率（DNA单链断裂的一种测量方法）。根据糖蛋白值高于或低于9%再将患者分为两组。糖蛋白值为12.9 +/- 2.4%（平均+/- SD）组的淋巴细胞DNA解绕速度较对照组加快（P < 0.01），而糖蛋白值为7.4 +/- 1.5%组的淋巴细胞DNA解绕速度较对照组加快（P < 0.01）。这些研究表明，控制不良的糖尿病可能导致DNA损伤，其对长期并发症的影响值得研究。
Certain aspects of the chronic complications of diabetes suggest that, with time, the abnormal metabolic milieu leads to irreversible changes in some cell populations. Since we have previously observed that high glucose concentrations induce an increase in single strand breaks in the DNA of cultured human endothelial cells, we have investigated whether the same abnormality occurs in cells derived from the in vivo diabetic environment. Peripheral blood lymphocytes obtained from 21 type I diabetic patients and age- and sex-matched controls were tested for rate of unwinding in alkali (a measure of DNA single strand breaks). The patients were subdivided into two groups on the basis of glycohemoglobin values above or below 9%. The group with glycohemoglobin values of 12.9 +/- 2.4% (mean +/- SD), but not the group with glycohemoglobin values of 7.4 +/- 1.5%, showed accelerated unwinding of lymphocyte DNA when compared to controls (P less than 0.01). These studies suggest that poorly controlled diabetes may result in DNA lesions, whose impact on long-term complications deserves to be investigated.
DOI：
10.1073/pnas.81.1.105
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BUCALA, R;MODEL, P;CERAMI, A
通讯作者：
CERAMI, A
DOI：
10.1073/pnas.83.7.2157
发表时间：
1986-04-01
影响因子：
11.1
作者：
SCHIMKE, RT;SHERWOOD, SW;JOHNSTON, RN
通讯作者：
JOHNSTON, RN
细胞氧化损伤。
DOI：
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发表时间：
1987
期刊：
International journal of tissue reactions
影响因子：
--
作者：
Schraufstatter,IU;Hyslop,PA;Jackson,J;Cochrane,CC
通讯作者：
Cochrane,CC