A gene regulatory network for neural induction.
A gene regulatory network for neural induction.
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DOI：
10.7554/elife.73189
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发表时间：
2023-03-03
期刊：
影响因子：
7.7
通讯作者：
Stern CD
中科院分区：
文献类型：
--
作者：
Trevers KE;Lu HC;Yang Y;Thiery AP;Strobl AC;Anderson C;Pálinkášová B;de Oliveira NMM;de Almeida IM;Khan MAF;Moncaut N;Luscombe NM;Dale L;Streit A;Stern CD
在脊椎动物早期发育过程中，来自胚胎特殊区域（组织者）的信号可以改变非神经外胚层细胞的命运，形成完整的模式化神经系统。这被称为神经诱导，通常被想象为一个单一的信号事件，导致命运的转变。在这里，我们进行了全面的分析，在非常精细的时间过程中，事件后的主管外胚层的小鸡的组织者（尖端的原始条纹，亨森的节点）曝光。使用转录组学和表观基因组学，我们产生了一个基因调控网络，包括175个转录调控因子和5614预测它们之间的相互作用，从最初暴露于成熟的神经板标记物的表达的信号与精细的时间动态。使用原位杂交，单细胞RNA测序和报告分析，我们表明，基因调控层次的反应嫁接组织者密切类似的事件正常神经板的发展。这项研究伴随着广泛的资源，包括有关其他脊椎动物中预测增强子的保护信息。
During early vertebrate development, signals from a special region of the embryo, the organizer, can redirect the fate of non-neural ectoderm cells to form a complete, patterned nervous system. This is called neural induction and has generally been imagined as a single signalling event, causing a switch of fate. Here, we undertake a comprehensive analysis, in very fine time course, of the events following exposure of competent ectoderm of the chick to the organizer (the tip of the primitive streak, Hensen’s node). Using transcriptomics and epigenomics we generate a gene regulatory network comprising 175 transcriptional regulators and 5614 predicted interactions between them, with fine temporal dynamics from initial exposure to the signals to expression of mature neural plate markers. Using in situ hybridization, single-cell RNA-sequencing, and reporter assays, we show that the gene regulatory hierarchy of responses to a grafted organizer closely resembles the events of normal neural plate development. The study is accompanied by an extensive resource, including information about conservation of the predicted enhancers in other vertebrates.
影响因子：
1.5
作者：
Khan, Mohsin A. F.;Soto-Jimenez, Luz Mayela;Howe, Timothy;Streit, Andrea;Sosinsky, Alona;Stern, Claudio D.
通讯作者：
Stern, Claudio D.
影响因子：
3.7
作者：
Pinho S;Simonsson PR;Trevers KE;Stower MJ;Sherlock WT;Khan M;Streit A;Sheng G;Stern CD
通讯作者：
Stern CD