Pigmented guinea pig skin irradiated with Q-switched ruby laser pulses. Morphologic and histologic findings.
Pigmented guinea pig skin irradiated with Q-switched ruby laser pulses. Morphologic and histologic findings.
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用 Q 开关红宝石激光脉冲照射有色素的豚鼠皮肤。
DOI：
10.1001/archderm.1989.01670130045004
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发表时间：
1989
影响因子：
--
通讯作者：
R. Anderson
中科院分区：
文献类型：
--
作者：
J. Dover;R. Margolis;L. Polla;Shinichi Watanabe;G. Hruza;J. Parrish;R. Anderson
Q开关红宝石激光脉冲对皮肤色素细胞造成选择性损伤。这种选择性损伤的修复还没有得到很好的描述。因此，我们使用脱毛色素和白化病豚鼠皮肤，研究了急性损伤和组织修复所造成的40纳秒，Q开关红宝石激光脉冲。进行大体观察、光镜和电镜检查。在白化病豚鼠中无明显特异性变化。在有色动物中，辐射暴露量为0.4 J/cm 2或更高时，局限于2.5 mm暴露部位的白色斑点立即形成，并在20分钟内消退。迟发性色素脱失发生在7 ~ 10天，随后在4 ~ 8周完全恢复色素沉着。在0.4 J/cm 2及以上剂量下照射的部位，再生毛发保持白色至少4个月。在组织学上，在0.3 J/cm 2及以上的暴露量下，在表皮和滤泡上皮中立即观察到充满色素的细胞空泡化。在0.3 J/cm 2及以上时，通过电子显微镜立即观察到黑色素体破坏。在接下来的7天内，表皮坏死后，色素脱失的表皮再生。到4个月时，黑素体和黑色素沉着已经恢复;然而，毛囊仍然脱色，没有黑素细胞。这项研究表明，Q开关红宝石激光脉冲引起的选择性黑色素体破坏导致短暂的皮肤色素脱失和持续的毛囊色素脱失。这种方法有可能用于选择性治疗色素沉着的表皮和真皮病变。
Q-switched ruby laser pulses cause selective damage to cutaneous pigmented cells. Repair of this selective damage has not been well described. Therefore, using epilated pigmented and albino guinea pig skin, we studied the acute injury and tissue repair caused by 40-ns, Q-switched ruby laser pulses. Gross observation and light and electron microscopy were performed. No specific changes were evident in the albino guinea pigs. In pigmented animals, with radiant exposures of 0.4 J/cm2 or greater, white spots confined to the 2.5-mm exposure sites developed immediately and faded over 20 minutes. Delayed depigmentation occurred at seven to ten days, followed by full repigmentation by four to eight weeks. Regrowing hairs in sites irradiated at and above 0.4 J/cm2 remained white for at least four months. Histologically, vacuolation of pigment-laden cells was seen immediately in the epidermis and the follicular epithelium at exposures of 0.3 J/cm2 and greater. Melanosomal disruption was seen immediately by electron microscopy at and above 0.3 J/cm2. Over the next seven days, epidermal necrosis was followed by regeneration of a depigmented epidermis. By four months, melanosomes and melanin pigmentation had returned; however, hair follicles remained depigmented and devoid of melanocytes. This study demonstrates that selective melanosomal disruption caused by Q-switched ruby laser pulses leads to transient cutaneous depigmentation and persistent follicular depigmentation. Potential exists for selective treatment of pigmented epidermal and dermal lesions with this modality.
影响因子：
6.5
作者：
ANDERSON, RR;PARRISH, JA
通讯作者：
PARRISH, JA