Over-Expression of RNA Processing, Heat Shock, and DNA Repair Proteins in Breast Tumor Compared to Normal Tissue.
Over-Expression of RNA Processing, Heat Shock, and DNA Repair Proteins in Breast Tumor Compared to Normal Tissue.
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与正常组织相比，乳腺肿瘤中 RNA 加工、热激和 DNA 修复蛋白的过度表达。
DOI：
10.1002/pmic.202000044
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发表时间：
2020-08
期刊：
影响因子：
3.4
通讯作者：
Timpe LC
中科院分区：
文献类型：
--
作者：
Yen TY;Wong R;Pizzo D;Thein M;Macher BA;Timpe LC
这项研究确定了与正常乳腺组织相比，人类乳腺肿瘤中蛋白质表达的主要变化。使用 LC/MS/MS 对来自甲醛固定、石蜡包埋标本的恶性肿瘤 (32) 和正常乳腺组织样本 (23) 进行发现蛋白质组学分析，并使用光谱计数进行定量。该数据集包含 1406 个蛋白质。使用利用载玻片上肿瘤百分比估计的方法来测量差异表达。该分析表明，肿瘤过度表达的主要蛋白质类别是 RNA 结合蛋白、热休克蛋白和 DNA 修复蛋白。 RNA 结合蛋白、HNRNP、SRSF 和延伸因子构成了最大的一组。与另一项研究结果的比较表明，RNA 结合蛋白与恶性转化特异性相关，而不是与细胞增殖相关。 HNRNP 和 SRSF 蛋白有助于定义正常细胞中的剪接位点。它们的过度表达可能会导致剪接失调，从而有可能促进恶性转化。
This study identifies the main changes in protein expression in human breast tumors compared to normal breast tissue. Malignant tumors (32) and normal breast tissue samples (23), from formaldehyde-fixed, paraffin-embedded specimens were subjected to discovery proteomics using LC/MS/MS, with spectral counts for quantitation. The dataset contains 1406 proteins. Differential expression was measured using a method that takes advantage of estimates of the percentage of tumor on a slide. This analysis shows that the major classes of proteins over-expressed by tumors are RNA-binding, heat shock and DNA repair proteins. RNA-binding proteins, HNRNPs, SRSF and elongation factors form the largest group. Comparison with results from another study demonstrate that the RNA-binding proteins are associated specifically with malignant transformation, rather than with cell proliferation. HNRNP and SRSF proteins help define splice sites in normal cells. Their over-expression may dysregulate splicing, which in turn has the potential to promote malignant transformation.
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替代RNA剪接和癌症。
影响因子：
7.3
作者：
Liu, Sali;Cheng, Chonghui
通讯作者：
Cheng, Chonghui
影响因子：
4.4
作者：
Alkhas, Addie;Hood, Brian L.;Conrads, Thomas P.
通讯作者：
Conrads, Thomas P.
影响因子：
3.8
作者：
Piehowski PD;Petyuk VA;Sontag RL;Gritsenko MA;Weitz KK;Fillmore TL;Moon J;Makhlouf H;Chuaqui RF;Boja ES;Rodriguez H;Lee JSH;Smith RD;Carrick DM;Liu T;Rodland KD
通讯作者：
Rodland KD
DOI：
10.1038/nrc.2016.51
发表时间：
2016-07
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
--
影响因子：
64.8
作者：
Mertins, Philipp;Mani, D. R.;Ruggles, Kelly V.;Gillette, Michael A.;Clauser, Karl R.;Wang, Pei;Wang, Xianlong;Qiao, Jana W.;Cao, Song;Petralia, Francesca;Kawaler, Emily;Mundt, Filip;Krug, Karsten;Tu, Zhidong;Lei, Jonathan T.;Gatza, Michael L.;Wilkerson, Matthew;Perou, Charles M.;Yellapantula, Venkata;Huang, Kuan-lin;Lin, Chenwei;McLellan, Michael D.;Yan, Ping;Davies, Sherri R.;Townsend, R. Reid;Skates, Steven J.;Wang, Jing;Zhang, Bing;Kinsinger, Christopher R.;Mesri, Mehdi;Rodriguez, Henry;Ding, Li;Paulovich, Amanda G.;Fenyo, David;Ellis, Matthew J.;Carr, Steven A.
通讯作者：
Carr, Steven A.