Genome-wide analysis of wheat calcium ATPases and potential role of selected ACAs and ECAs in calcium stress.
Genome-wide analysis of wheat calcium ATPases and potential role of selected ACAs and ECAs in calcium stress.
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DOI：
10.1186/s12870-017-1112-5
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发表时间：
2017-10-27
影响因子：
5.3
通讯作者：
Virk N
中科院分区：
文献类型：
--
作者：
Aslam R;Williams LE;Bhatti MF;Virk N
P2型钙ATP酶（ACAs-autoinhibited calcium ATPases和ECAs-endoplasmic reticulum calcium ATPases）属于P型ATP酶家族的活性膜转运蛋白，在维持细胞质中Ca ~（2+）、Mn ~（2+）和Zn ~（2+）的稳定水平、逆境信号传导、气孔开闭和花粉管生长等方面发挥重要作用。在这里，我们报告的鉴定和可能的作用，这些ATP酶从小麦。本研究从不同的数据库中检索了禾本科6个物种的ACA和ECA序列，并构建了系统发育树。高度的进化相关性之间观察到P2序列的特点，在这项研究中。来自不同植物物种的相应组的成员被观察到属于同一分支。这种模式突出了P2−型钙ATP酶的共同祖先。此外，qRT-PCR用于分析来自小麦（Triticum aestivum）（小麦）的所选ACA和ECA在钙毒害和钙缺乏下的表达。这些数据表明，ECAs的表达增强钙胁迫下，这些ATP酶在小麦钙稳态的可能作用。类似地，ACA的表达在钙胁迫下生长的植物中与在对照条件下生长的植物相比显著不同。这为ACAs在小麦钙胁迫信号转导中的作用提供了线索。小麦基因组由9个P2B型和3个P2 A型钙ATP酶组成。此外，小麦祖先中的基因丢失事件导致小麦中某一基因的特定同源物的丢失。为了阐明这些小麦ATP酶的作用，进行qRT-PCR。结果表明，当植物受到钙胁迫时，P2 A和P2B基因的表达均增强。这进一步提供了小麦中这些ATP酶在钙管理中的可能作用的线索。这些发现可用于未来的遗传操作以及小麦基因组注释过程中。
P2- type calcium ATPases (ACAs-auto inhibited calcium ATPases and ECAs-endoplasmic reticulum calcium ATPases) belong to the P- type ATPase family of active membrane transporters and are significantly involved in maintaining accurate levels of Ca2+, Mn2+ and Zn2+ in the cytosol as well as playing a very important role in stress signaling, stomatal opening and closing and pollen tube growth. Here we report the identification and possible role of some of these ATPases from wheat. In this study, ACA and ECA sequences of six species (belonging to Poaceae) were retrieved from different databases and a phylogenetic tree was constructed. A high degree of evolutionary relatedness was observed among P2 sequences characterized in this study. Members of the respective groups from different plant species were observed to fall under the same clade. This pattern highlights the common ancestry of P2− type calcium ATPases. Furthermore, qRT-PCR was used to analyse the expression of selected ACAs and ECAs from Triticum aestivum (wheat) under calcium toxicity and calcium deficiency. The data indicated that expression of ECAs is enhanced under calcium stress, suggesting possible roles of these ATPases in calcium homeostasis in wheat. Similarly, the expression of ACAs was significantly different in plants grown under calcium stress as compared to plants grown under control conditions. This gives clues to the role of ACAs in signal transduction during calcium stress in wheat. Here we concluded that wheat genome consists of nine P2B and three P2A -type calcium ATPases. Moreover, gene loss events in wheat ancestors lead to the loss of a particular homoeolog of a gene in wheat. To elaborate the role of these wheat ATPases, qRT-PCR was performed. The results indicated that when plants are exposed to calcium stress, both P2A and P2B gene expression get enhanced. This further gives clues about the possible role of these ATPases in wheat in calcium management. These findings can be useful in future for genetic manipulations as well as in wheat genome annotation process.
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