Rnd3-induced cell rounding requires interaction with Plexin-B2.
Rnd3-induced cell rounding requires interaction with Plexin-B2.
复制标题
DOI：
10.1242/jcs.192211
复制
发表时间：
2016-11-01
影响因子：
4
通讯作者：
Ridley AJ
中科院分区：
文献类型：
--
作者：
McColl B;Garg R;Riou P;Riento K;Ridley AJ
Rnd蛋白是Rho GTPase家族的非典型成员，可诱导肌动蛋白细胞骨架重组和细胞圆缩。据报道，Rnd蛋白与几种丛蛋白受体的细胞内结构域结合，但丛蛋白是否参与了Rnd诱导的舍入反应尚不清楚。我们发现Rnd3优先与三种丛状蛋白b中的丛状蛋白b2相互作用，而Rnd2与所有三种b型丛状蛋白相互作用，而Rnd1在免疫沉淀中仅与丛状蛋白b表现出非常弱的相互作用。据报道，Plexin-B1可作为R-Ras和/或Rap1蛋白的GAP。我们发现所有三种丛状蛋白b都与R-Ras和Rap1相互作用，但Rnd蛋白不改变这种相互作用或R-Ras或Rap1活性。我们证明丛状蛋白b2促进Rnd3诱导的细胞圆缩和应力纤维的丢失，并增强了Rnd3对HeLa细胞侵袭的抑制作用。我们确定了Rnd3中丛蛋白- b2相互作用所需的氨基酸，并表明这些氨基酸的突变可以阻止Rnd3诱导的形态变化。这些结果表明plexin-B2是Rnd3的下游靶点，参与了Rnd3的细胞功能。摘要：Rho GTPase Rnd3可以改变细胞形状和肌动蛋白细胞骨架。在这里，信号素受体丛蛋白b2被鉴定为一个新的Rnd3伙伴，介导其对细胞形状的影响。
Rnd proteins are atypical members of the Rho GTPase family that induce actin cytoskeletal reorganization and cell rounding. Rnd proteins have been reported to bind to the intracellular domain of several plexin receptors, but whether plexins contribute to the Rnd-induced rounding response is not known. Here we show that Rnd3 interacts preferentially with plexin-B2 of the three plexin-B proteins, whereas Rnd2 interacts with all three B-type plexins, and Rnd1 shows only very weak interaction with plexin-B proteins in immunoprecipitations. Plexin-B1 has been reported to act as a GAP for R-Ras and/or Rap1 proteins. We show that all three plexin-B proteins interact with R-Ras and Rap1, but Rnd proteins do not alter this interaction or R-Ras or Rap1 activity. We demonstrate that plexin-B2 promotes Rnd3-induced cell rounding and loss of stress fibres, and enhances the inhibition of HeLa cell invasion by Rnd3. We identify the amino acids in Rnd3 that are required for plexin-B2 interaction, and show that mutation of these amino acids prevents Rnd3-induced morphological changes. These results indicate that plexin-B2 is a downstream target for Rnd3, which contributes to its cellular function. Summary: The Rho GTPase Rnd3 is known to alter cell shape and the actin cytoskeleton. Here, the semaphorin receptor plexin-B2 is identified as a new Rnd3 partner that mediates its effects on cell shape.
登录
查看更多内容
影响因子：
4.5
作者：
Clarke K;Daubon T;Turan N;Soulet F;Mohd Zahari M;Ryan KR;Durant S;He S;Herbert J;Ankers J;Heath JK;Bjerkvig R;Bicknell R;Hotchin NA;Bikfalvi A;Falciani F
通讯作者：
Falciani F
DOI：
10.1042/bj20082377
发表时间：
2009-10-23
期刊：
The Biochemical journal
影响因子：
--
作者：
Madigan JP;Bodemann BO;Brady DC;Dewar BJ;Keller PJ;Leitges M;Philips MR;Ridley AJ;Der CJ;Cox AD
通讯作者：
Cox AD
影响因子：
3.7
作者：
Mocholí E;Ballester-Lurbe B;Arqué G;Poch E;Peris B;Guerri C;Dierssen M;Guasch RM;Terrado J;Pérez-Roger I
通讯作者：
Pérez-Roger I
影响因子：
2.4
作者：
Gottesbühren U;Garg R;Riou P;McColl B;Brayson D;Ridley AJ
通讯作者：
Ridley AJ
影响因子：
3.5
作者：
McKenzie, JAG;Riento, K;Ridley, AJ
通讯作者：
Ridley, AJ