Bipolar nanosecond electric pulses are less efficient at electropermeabilization and killing cells than monopolar pulses.
Bipolar nanosecond electric pulses are less efficient at electropermeabilization and killing cells than monopolar pulses.
复制标题
DOI：
10.1016/j.bbrc.2013.12.004
复制
发表时间：
2014-01-10
影响因子：
3.1
通讯作者：
Pakhomov, Andrei G.
中科院分区：
文献类型：
--
作者：
Ibey, Bennett L.;Ullery, Jody C.;Pakhomova, Olga N.;Roth, Caleb C.;Semenov, Iurii;Beier, Hope T.;Tarango, Melissa;Xiao, Shu;Schoenbach, Karl H.;Pakhomov, Andrei G.
多项研究表明，与单极（MP）脉冲相比，微秒范围内的双极（BP）电脉冲在渗透细胞时更有效，同时保持相似的细胞存活率。在本文中，我们研究了BP纳秒脉冲电场（nsPEF）与MP纳秒脉冲电场（nsPEF）是否存在相同的优势。为了研究其通透性，用共聚焦显微镜观察了MP或BP脉冲对单只中国仓鼠卵巢（CHO）细胞的渗透效果，并测定了三种染料（钙绿-1、碘化丙啶（PI）和FM1-43）的反应。结果表明，与MP脉冲相比，BP脉冲在增加细胞内钙浓度或PI摄取方面的效果较差，并且引起的膜重组（FM1-43）较少。在三个细胞系（Jurkat, U937， CHO）中测量了24小时的存活率，与相同强度和持续时间的MP脉冲相比，诱导死亡所需的BP脉冲超过10倍。暴露后（15分钟）CHO细胞的流式细胞术分析显示，要实现FITC-Annexin V和PI的阳性表达，所需的BP脉冲是MP脉冲的10倍。总的来说，与较长脉冲暴露不同，BP nsPEF暴露在膜渗透和细胞杀伤方面的效果远不如MP nsPEF。
Multiple studies have shown that bipolar (BP) electric pulses in the microsecond range are more effective at permeabilizing cells while maintaining similar cell survival rates as compared to monopolar (MP) pulse equivalents. In this paper, we investigated whether the same advantage existed for BP nanosecond-pulsed electric fields (nsPEF) as compared to MP nsPEF. To study permeabilization effectiveness, MP or BP pulses were delivered to single Chinese hamster ovary (CHO) cells and the response of three dyes, Calcium Green-1, Propidium Iodide (PI), and FM1-43, was measured by confocal microscopy. Results show that BP pulses were less effective at increasing intracellular calcium concentration or PI uptake and cause less membrane reorganization (FM1-43) than MP pulses. Twenty-four hour survival was measured in three cell lines (Jurkat, U937, CHO) and over ten times more BP pulses were required to induce death as compared to MP pulses of similar magnitude and duration. Flow cytometry analysis of CHO cells after exposure (15 minutes) revealed that to achieve positive FITC-Annexin V and PI expression, ten times more BP pulses were required than MP pulses. Overall, unlike longer pulse exposures, BP nsPEF exposures proved far less effective at both membrane permeabilization and cell killing than MP nsPEF.
登录
查看更多内容
影响因子：
4
作者：
Semenov I;Xiao S;Pakhomova ON;Pakhomov AG
通讯作者：
Pakhomov AG
影响因子：
3.9
作者：
Arena CB;Sano MB;Rossmeisl JH Jr;Caldwell JL;Garcia PA;Rylander MN;Davalos RV
通讯作者：
Davalos RV
影响因子：
4.6
作者：
Arena, Christopher B.;Sano, Michael B.;Davalos, Rafael V.
通讯作者：
Davalos, Rafael V.
影响因子：
1.9
作者：
Schoenbach, KH;Beebe, SJ;Buescher, ES
通讯作者：
Buescher, ES
影响因子：
--
作者：
Vernier, P. Thomas;Sun, Yinghua;Gundersen, Martin A.
通讯作者：
Gundersen, Martin A.