Assessing the in vivo efficacy of doxorubicin loaded hyaluronan nanoparticles.
Assessing the in vivo efficacy of doxorubicin loaded hyaluronan nanoparticles.
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DOI：
10.1021/am404946v
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发表时间：
2014-01-08
影响因子：
9.5
通讯作者：
Huang, Xuefei
中科院分区：
文献类型：
--
作者：
El-Dakdouki, Mohammad H.;Xia, Jingguang;Zhu, David C.;Kavunja, Herbert;Grieshaber, Jessica;O'Reilly, Sandra;McCormick, J. Justin;Huang, Xuefei
磁性纳米颗粒是生物医学应用的有吸引力的平台，包括疾病的诊断和治疗。我们以前已经表明，透明质酸包被的超顺磁性氧化铁纳米粒子（HA-SPION）增强了体外抗癌药物阿霉素（DOX）对药物敏感和耐药人卵巢癌细胞的疗效。在本文中，我们报告了我们在裸鼠皮下和腹腔内SKOV-3卵巢肿瘤模型中对DOX负载HA-SPION纳米颗粒的体内疗效的发现。通过磁共振成像证实了静脉注射纳米颗粒后纳米颗粒在皮下肿瘤中的积聚，并通过共聚焦显微镜和普鲁士蓝染色评估了其在肿瘤中的分布。通过纳米颗粒递送的DOX在肿瘤组织中以比静脉内注射的游离DOX高得多的水平积累并且分布更广，导致肿瘤生长的显著减少。用于体内生物发光成像的IVIS光谱用于辅助对由表达萤火虫荧光素酶的人卵巢SKOV-3细胞形成的腹膜内卵巢肿瘤的DOX负载的纳米颗粒的治疗评估。DOX负载的HA-SPION显著减少肿瘤生长，延迟肿瘤发展，并延长小鼠的存活期。因此，利用HA-SPION作为药物递送载体构成了解决表达CD 44的卵巢癌的有希望的方法。
Magnetic nanoparticles are attractive platforms for biomedical applications including diagnosis and treatment of diseases. We have shown previously that hyaluronan-coated superparamagnetic iron oxide nanoparticles (HA-SPION) enhanced the efficacy of the conjugated anticancer drug doxorubicin (DOX) in vitro against drug-sensitive and drug-resistant human ovarian cancer cells. In this manuscript, we report our findings on the efficacy of DOX loaded HA-SPION nanoparticles in vivo using subcutaneous and intraperitoneal SKOV-3 ovarian tumor models in nude mice. The accumulation of the nanoparticles in subcutaneous tumors following an intravenous nanoparticle administration was confirmed by magnetic resonance imaging, and its distribution in the tumors was evaluated by confocal microscopy and Prussian blue staining. DOX delivered by nanoparticles accumulated at much higher levels and distributed wider in the tumor tissue than intravenously injected free DOX leading to significant reduction of tumor growth. The IVIS Spectrum for in vivo bioluminescence imaging was used to aid in therapy assessment of the DOX-loaded nanoparticles on intraperitoneal ovarian tumors formed by firefly Luciferase expressing human ovarian SKOV-3 cells. DOX-loaded HA-SPIONs significantly reduced tumor growth, delayed tumor development, and extended the survival of mice. Thus, utilizing HA-SPIONs as drug delivery vehicles constitutes a promising approach to tackle CD44 expressing ovarian cancer.
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影响因子：
6.7
作者：
El-Dakdouki MH;Puré E;Huang X
通讯作者：
Huang X
影响因子：
6.7
作者：
El-Dakdouki MH;Puré E;Huang X
通讯作者：
Huang X
DOI：
10.1038/nrc2644
发表时间：
2009-06
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
--
影响因子：
--
作者：
Markman M;Sehouli J;Levenback CF;Chi DS
通讯作者：
Chi DS
影响因子：
4.7
作者：
GORE, ME;FRYATT, I;DAWSON, T
通讯作者：
DAWSON, T