A direct circular dichroic assay for quantitative determination of peptide enantiomers.
A direct circular dichroic assay for quantitative determination of peptide enantiomers.
复制标题
用于定量测定肽对映体的直接圆二色测定。
DOI：
10.1093/abbs/gmab104
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发表时间：
2021-08
期刊：
影响因子：
--
通讯作者：
Ming Peng
中科院分区：
文献类型：
--
作者：
Jie Yang;Shuai Zhong;Xiu-Lan Chen;Yang Yu;Yu-Zhong Zhang;Hai-Nan Su;Xiao-Yan Song;Ming Peng
除了甘氨酸外，氨基酸有两种类型的分子结构，D-和L-型，它们是对映体，并显示出不同的生理和生物活性。它们进一步形成更高级的分子，如肽和蛋白质。肽聚糖是在天然生物结构组成中存在D-氨基酸的最佳研究实例之一。氨基酸和肽，如D-丙氨酸（D-Ala）、D-谷氨酸（D-Glu）和L-丙氨酰-D-谷氨酸（L-Ala-D-Glu），是细菌细胞壁中肽聚糖层的重要组成部分，有助于其抵抗肽酶水解[1]。催化丙氨酸对映体消旋化的丙氨酸消旋酶和催化L-Ala-D/L-Glu等二肽差向异构化的L-Ala-D/L-Glu差向异构酶是肽聚糖合成和再循环的必需酶[2，3]，L-氨基酸和肽在大多数情况下对生物系统起积极作用，而D-氨基酸和肽可能表现出不利影响。D-氨基酸的存在导致肽键的扭曲和蛋白质的异常缩合，这可能导致衰老疾病[4]。生物样品中氨基酸和肽的对映体比率可用于生物测年应用[5]。由于L-型氨基酸和肽通常是工业生产中所需的产物[6]，因此控制其光学纯度很重要，因此需要开发一种方法来确定D-型分子的浓度。
Amino acids, except glycine, have two types of molecular structures, D-and L-forms, which are enantiomers and show different physiological and biological activities. They further form higher order molecules such as peptides and proteins. Peptidoglycan is one of the best-studied examples of the presence of D-amino acids in the composition of natural biological structure. Amino acids and peptides, such as D-alanine (D-Ala), D-glutamate (D-Glu), and L-alanyl-D-Glutamate (L-Ala-D-Glu), are important components of the peptidoglycan layer in cell walls of bacteria, contributing to their resistance to hydrolysis by peptidases [1]. Alanine racemase that catalyzes the racemization of both alanine enantiomers and L-Ala-D/L-Glu epimerase that catalyzes the epimerization of L-Ala-D/L-Glu and other dipeptides are essential enzymes for the synthesis and recycling of peptidoglycan [2, 3].L-amino acids and peptides play positive roles in biological systems in most cases, while D-amino acids and peptides may show adverse effects. The presence of D-amino acids leads to a distortion of peptide bonds and an abnormal condensation of proteins, which can cause aging diseases [4]. The enantiomeric ratio of amino acids and peptides within a biological sample can be used in biological dating applications [5]. Due to the fact that the L-form of amino acids and peptides are usually the desired products in industrial production [6], it is important to control their optical purity, and therefore, there is a need to develop a method to determine the concentration of D-form molecules.
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影响因子：
3.5
作者：
G. Lubec;B. Lubec
通讯作者：
G. Lubec;B. Lubec
DOI：
--
发表时间：
1989-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
C. Walsh
通讯作者：
C. Walsh
影响因子：
4.8
作者：
Noda, M;Kawahara, Y;Sugiyama, M
通讯作者：
Sugiyama, M
影响因子：
4.1
作者：
Noda, M;Matoba, Y;Sugiyama, M
通讯作者：
Sugiyama, M
影响因子：
1.1
作者：
Chernobrovkin, MG;Anan'eva, IA;Shpigun, OA
通讯作者：
Shpigun, OA