Alfacalcidol enhances collagen quality in ovariectomized rat bones.
Alfacalcidol enhances collagen quality in ovariectomized rat bones.
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DOI：
10.1002/jor.22642
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发表时间：
2014-08
影响因子：
2.8
通讯作者：
Yamauchi, Mitsuo
中科院分区：
文献类型：
--
作者：
Nagaoka, Hideaki;Terajima, Masahiko;Yamada, Shizuka;Azuma, Yoshiaki;Chida, Takayuki;Yamauchi, Mitsuo
本研究的目的是探讨阿法骨化醇（1α（OH）D3：ALF）对骨胶原的影响，采用去卵巢大鼠模型。将35只16周龄雌性Sprague-Dawley大鼠分为5组：SHAM（假手术+溶剂）、OVX（卵巢切除术+溶剂）和3个ALF给药组，即卵巢切除术+ 0.022 µg/kg/天ALF、卵巢切除术+ 0.067 µg/kg/天ALF和卵巢切除术+ 0.2 µg/kg/天ALF。治疗12周后，进行组织学、生化和免疫组织化学分析。OVX骨中的胶原基质表现为不成熟和组织不良;然而，ALF治疗后，其以剂量依赖性方式得到改善。胶原和吡啶啉交联的含量在OVX组较SHAM组降低，但在ALF治疗组则升高至与SHAM相当的水平。最高剂量ALF中的总醛，即游离醛和涉及交联的醛的总和，显著高于其余组（p < 0.05）。此外，与OVX相比，ALF处理组中赖氨酰氧化酶的表达增加（p < 0.05）。总之，ALF不仅增加了胶原蛋白的量，而且还促进了卵巢切除诱导的骨质疏松骨中胶原蛋白的成熟，这可能有助于改善骨质量。© 2014作者。骨科研究杂志。出版社：Wiley Periodicals，Inc. J Orthop Res 32：1030-1036，2014.
The aim of this study was to investigate the effects of alfacalcidol (1α(OH)D3: ALF) on bone collagen employing an ovariectomized rat model. Thirty-five 16-week-old female Sprague-Dawley rats were divided into five groups: SHAM (sham-operated + vehicle), OVX (ovariectomy + vehicle), and three ALF-treated groups, that is, ovariectomy + 0.022 µg/kg/day ALF, ovariectomy + 0.067 µg/kg/day ALF, and ovariectomy + 0.2 µg/kg/day ALF. After 12 weeks of treatment, tibiae were subjected to histological, biochemical and immunohistochemical analyses. Collagen matrices in OVX bone appeared as immature and poorly organized; however, with the ALF treatment, it was improved in a dose-dependent manner. Contents of collagen and pyridinoline cross-link were decreased in OVX compared with SHAM, but they increased to the level comparable to SHAM in ALF-treated groups. The total aldehyde, that is, a sum of free and those involved cross-links, in the highest dose of ALF was significantly higher than the rest of the groups (p < 0.05). In addition, the expression of lysyl oxidase was increased in the all ALF-treated groups compared with OVX (p < 0.05). In conclusion, ALF increases not only the amount of collagen but also enhances the maturation of collagen in ovariectomy-induced osteoporotic bones, which likely contributes to the improvement of bone quality. © 2014 The Authors. Journal of Orthopaedic Research. Published by Wiley Periodicals, Inc. J Orthop Res 32:1030–1036, 2014.
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影响因子：
4.8
作者：
HARRISON, JR;PETERSEN, DN;KREAM, BE
通讯作者：
KREAM, BE
DOI：
10.1073/pnas.96.3.1054
发表时间：
1999-02-02
影响因子：
11.1
作者：
Bank, RA;Robins, SP;TeKoppele, JM
通讯作者：
TeKoppele, JM
影响因子：
6.2
作者：
Pornprasertsuk, S;Duarte, WR;Yamauchi, M
通讯作者：
Yamauchi, M
影响因子：
56.9
作者：
HOLICK, MF;SEMMLER, EJ;DELUCA, HF
通讯作者：
DELUCA, HF
影响因子：
4.2
作者：
Shiraishi, A;Higashi, S;Nakamura, T
通讯作者：
Nakamura, T