Efficient screening of adsorbed receptors for Salmonella phage LP31 and identification of receptor-binding protein.
Efficient screening of adsorbed receptors for Salmonella phage LP31 and identification of receptor-binding protein.
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DOI：
10.1128/spectrum.02604-23
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发表时间：
2023-09-20
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
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吸附过程是噬菌体生命周期的第一步，在整个感染过程中起着决定性作用。确定噬菌体的吸附机制不仅可以使噬菌体治疗更加精确和高效，而且可以探索噬菌体的其他潜在应用和修饰。噬菌体LP31可以裂解多种血清型沙门氏菌，有效清除肠道血清型肠炎沙门氏菌（S. Enteritidis）形成的生物膜，显著降低鸡粪便中肠炎沙门氏菌的浓度。因此，LP31具有很大的实际应用潜力。本研究建立了噬菌体感染相关基因的高效筛选方法，共鉴定出10个与噬菌体LP31吸附过程相关的基因。构建菌株C50041ΔrfaL 58−358后，发现敲除菌株具有o抗原缺陷菌株的粗略表型。吸附率和透射电镜实验表明，噬菌体LP31的受体为肠炎链球菌O9抗原。同源性比较和吸附实验证实，噬菌体LP31的尾纤维蛋白Lp35作为受体结合蛋白参与了吸附过程。充分了解噬菌体与其受体之间的相互作用有助于噬菌体相关产品的开发。据报道，具有尾纤维蛋白Lp35或密切相关蛋白的噬菌体LP31可以有效识别和感染多种沙门氏菌血清型。然而，这些蛋白在噬菌体感染中的作用以前没有被描述过。在本研究中，我们建立了一种检测噬菌体对宿主受体吸附的高效筛选方法。我们发现噬菌体LP31可以利用其尾部纤维蛋白Lp35吸附肠炎链球菌的O9抗原，启动感染过程。本研究为进一步研究噬菌体编码受体结合蛋白（RBP）如何与宿主RBP结合靶点相互作用提供了一个很好的模型体系，为进一步了解噬菌体的感染机制提供了理论和实验研究的机会。
The adsorption process is the first step in the lifecycle of phages and plays a decisive role in the entire infection process. Identifying the adsorption mechanism of phages not only makes phage therapy more precise and efficient but also enables the exploration of other potential applications and modifications of phages. Phage LP31 can lyse multiple Salmonella serotypes, efficiently clearing biofilms formed by Salmonella enterica serovar Enteritidis (S. Enteritidis) and significantly reducing the concentration of S. Enteritidis in chicken feces. Therefore, LP31 has great potential for many practical applications. In this study, we established an efficient screening method for phage infection-related genes and identified a total of 10 genes related to the adsorption process of phage LP31. After the construction of strain C50041ΔrfaL 58−358, it was found that the knockout strain had a rough phenotype as an O-antigen-deficient strain. Adsorption rate and transmission electron microscopy experiments showed that the receptor for phage LP31 was the O9 antigen of S. Enteritidis. Homology comparison and adsorption experiments confirmed that the tail fiber protein Lp35 of phage LP31 participated in the adsorption process as a receptor-binding protein. A full understanding of the interaction between phages and their receptors can help with the development of phage-related products. Phages like LP31 with the tail fiber protein Lp35, or a closely related protein, have been reported to effectively recognize and infect multiple Salmonella serotypes. However, the role of these proteins in phage infection has not been previously described. In this study, we established an efficient screening method to detect phage adsorption to host receptors. We found that phage LP31 can utilize its tail fiber protein Lp35 to adsorb to the O9 antigen of S. Enteritidis, initiating the infection process. This study provides a great model system for further studies of how a phage-encoded receptor-binding protein (RBP) interacts with its host's RBP binding target, and this new model offers opportunities for further theoretical and experimental studies to understand the infection mechanism of phages.
影响因子：
4.4
作者：
Zhang J;Luo W;Liu G;Wang Y;Geng S;Pan Z;Jiao X
通讯作者：
Jiao X
影响因子：
3.7
作者：
通讯作者：
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