High genetic similarity of Salmonella Enteritidis as a predominant serovar by an independent survey in 3 large-scale chicken farms in China.
High genetic similarity of Salmonella Enteritidis as a predominant serovar by an independent survey in 3 large-scale chicken farms in China.
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DOI：
10.1016/j.psj.2020.12.038
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发表时间：
2021-04
期刊：
影响因子：
4.4
通讯作者：
Jiao X
中科院分区：
文献类型：
--
作者：
Zhang J;Luo W;Liu G;Wang Y;Geng S;Pan Z;Jiao X
肠炎沙门氏菌(Salmonella Enteritidis，SE)是一种重要的人畜共患病病原体，容易通过受污染的动物产品传播给人类。现代规模化养鸡场有必要进行SE疫情调查。本研究从3个独立养殖场采集的可能感染沙门氏菌样品中分离到沙门氏菌，并采用多位点序列分型(MLST)、聚类规则间隔短回文重复序列(CRISPR)、脉冲场凝胶电泳(PFGE)和全基因组测序(WGS)等分子遗传分析技术对其血清型、耐药性、毒力基因和遗传相似性进行了分析。从3598份标本中分离出沙门氏菌346株(9.61%)，其中SE 329株(95.09%)，多重耐药308株(93.62%)。根据6个毒力基因进行的病毒分型，除2个分离物外，其余各猪场的SE分离物具有很高的相似性。MLST结果表明，所有SE分离株均为ST11型，随机选择的150株SE分离株中，有22株经PFGE鉴定为1类，CRISPR鉴定为相同的SET1型。WGS结果进一步表明，这些菌株属于同一克隆群，遗传相似度在99.80%~100.00%之间。所有这些结果表明，这些SE分离株具有压倒性的优势，并表现出高度的遗传相似性，这表明相同的SE克隆可能在这些农场传播。
Salmonella Enteritidis (SE) are important zoonotic pathogens, and can be easily transferred to humans by contaminated animal products. Epidemic surveys of SE are necessary in current modern large-scale chicken farms. In this study, Salmonella strains were isolated from possibly infected samples collected at 3 independent farms, and their serotype, drug resistances, virulence genes, and genetic similarity were analyzed by molecular genetic analysis technologies including multilocus sequence typing (MLST), clustered regularly interspaced short palindromic repeats (CRISPR), pulsed-field gel electrophoresis (PFGE), and whole-genome sequencing (WGS). A total of 346 Salmonella strains were isolated from 3,598 samples (9.61%); 329 isolates were identified as SE (95.09%) and 308 isolates were multidrug resistant (93.62%). Virulotyping based on 6 virulence genes showed high similarity in SE isolates of each farm, with the exception of 2 isolates. All SE isolates were found to be the same ST11 type by MLST, and 22 strains of 150 SE isolates selected at random were found to belong to 1 cluster by PFGE and the same SET1 type by CRISPR. WGS results further revealed that these isolates belonged to the same clonal cluster, with high genetic similarity of 99.80 to 100.00%. All these results indicated that these SE isolates were overwhelmingly dominant and demonstrated high genetic similarity, which revealed that the same SE clone might be transmitted in these farms.
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作者：
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DOI：
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