Tracking Escherichia coli DNA polymerase V to the entire genome during the SOS response.
Tracking Escherichia coli DNA polymerase V to the entire genome during the SOS response.
复制标题
DOI：
10.1016/j.dnarep.2021.103075
复制
发表时间：
2021-05
期刊：
影响因子：
3.8
通讯作者：
Clausen AR
中科院分区：
文献类型：
--
作者：
Faraz M;Woodgate R;Clausen AR
Ribonucleotides are frequently incorporated into DNA and can be used as a marker of DNA replication enzymology. To investigate on a genome-wide scale, how E. coli pol V accesses undamaged chromosomal DNA during the SOS response, we mapped the location of ribonucleotides incorporated by steric gate variants of pol V across the entire E. coli genome. To do so, we used strains that are deficient in ribonucleotide excision repair (ΔrnhB), deficient in pol IV DNA polymerase, constitutively express all SOS-regulated genes [lexA(Def)] and constitutively “activated” RecA* (recA730). The strains also harbor two steric gate variants of E. coli pol V (Y11A, or F10L), or a homolog of pol V, (pol VR391-Y13A). Ribonucleotides are frequently incorporated by the pol V-Y11A and pol VR391-Y13A variants, with a preference to the lagging strand. In contrast, the pol V-F10L variant incorporates less ribonucleotides and no strand preference is observed. Sharp transitions in strand specificity are observed at the replication origin (oriC), while a gradient is observed at the termination region. To activate RecA* in a recA+ strain, we treated the strains with ciprofloxacin and genome-wide mapped the location of the incorporated ribonucleotides. Again, the pol V-Y11A steric gate variant exhibited a lagging strand preference. Our data are consistent with a specific role for pol V in lagging strand DNA synthesis across the entire E. coli genome during the SOS response.
登录
查看更多内容
影响因子：
4.5
作者：
McDonald, John P.;Vaisman, Alexandra;Woodgate, Roger
通讯作者：
Woodgate, Roger
DOI：
10.1007/bf00267207
发表时间：
1980-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
OKA, A;SUGIMOTO, K;HIROTA, Y
通讯作者：
HIROTA, Y
DOI：
10.1007/978-1-4939-7306-4_23
发表时间：
2018-01-01
期刊：
GENOME INSTABILITY: METHODS AND PROTOCOLS
影响因子：
--
作者：
Orebaugh, Clinton D.;Lujan, Scott A.;Kunkel, Thomas A.
通讯作者：
Kunkel, Thomas A.
影响因子：
14.9
作者：
Henrikus, Sarah S.;Henry, Camille;Robinson, Andrew
通讯作者：
Robinson, Andrew
DOI：
10.1073/pnas.78.9.5749
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BAGG, A;KENYON, CJ;WALKER, GC
通讯作者：
WALKER, GC