Characterizing molecular diffusion in the lens capsule.
Characterizing molecular diffusion in the lens capsule.
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DOI：
10.1016/j.matbio.2009.12.004
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发表时间：
2010-04
期刊：
影响因子：
--
通讯作者：
Duncan MK
中科院分区：
文献类型：
--
作者：
Danysh BP;Patel TP;Czymmek KJ;Edwards DA;Wang L;Pande J;Duncan MK
透镜囊将无血管透镜的细胞与其他眼组织分隔开。透镜细胞代谢所需的小分子，如葡萄糖、盐和废物，可自由通过囊。然而，透镜囊可选择性地透过蛋白质，如生长激素和基质载体，这些是透镜正常生长和发育所需的。我们采用光漂白后荧光恢复法（FRAP）来表征不同大小的葡聚糖（3、10、40、150和250 kDa）和透镜环境内源性蛋白质（EGF、γ D-晶状体蛋白、BSA、转铁蛋白、血浆铜蓝蛋白和IgG）在整个活体晶状体囊内的扩散行为。我们发现，蛋白质有显着不同的扩散和分配系数，以及胶囊基质结合亲和力比类似大小的葡聚糖，但他们有可比的渗透性。我们还发现蛋白质和胶囊基质之间的离子相互作用显着影响渗透性和结合亲和力，而疏水相互作用的影响较小。从蛋白质γ D-晶状体蛋白[E107 A]表面去除单个阴离子残基，显著改变了其在胶囊中的渗透性和基质结合亲和力。我们的数据表明，在透镜囊的渗透性和结合亲和力不同的个别蛋白质，不能预测的等电点或分子大小。
The lens capsule compartmentalizes the cells of the avascular lens from other ocular tissues. Small molecules required for lens cell metabolism, such as glucose, salts, and waste products, freely pass through the capsule. However, the lens capsule is selectively permeable to proteins such as growth hormones and substrate carriers which are required for proper lens growth and development. We used fluorescence recovery after photobleaching (FRAP) to characterize the diffusional behavior of various sized dextrans (3, 10, 40, 150, and 250kDa) and proteins endogenous to the lens environment (EGF, γD-crystallin, BSA, transferrin, ceruloplasmin, and IgG) within the capsules of whole living lenses. We found that proteins had dramatically different diffusion and partition coefficients as well as capsule matrix binding affinities than similar sized dextrans, but they had comparable permeabilities. We also found ionic interactions between proteins and the capsule matrix significantly influence permeability and binding affinity, while hydrophobic interactions had less of an effect. The removal of a single anionic residue from the surface of a protein, γD-crystallin [E107A], significantly altered its permeability and matrix binding affinity in the capsule. Our data indicated that permeabilities and binding affinities in the lens capsule varied between individual proteins and cannot be predicted by isoelectric points or molecular size alone.
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DOI：
10.1111/j.1600-0420.2005.00381.x
发表时间：
2005-02-01
期刊：
ACTA OPHTHALMOLOGICA SCANDINAVICA
影响因子：
--
作者：
Funding, M;Vorum, H;Ehlers, N
通讯作者：
Ehlers, N
影响因子：
64.8
作者：
COTLIER, E;FOX, J;DUPREE, A
通讯作者：
DUPREE, A
影响因子：
3.4
作者：
Edwards, A;Deen, WM;Daniels, BS
通讯作者：
Daniels, BS
影响因子：
19.6
作者：
BRAY, J;ROBINSON, GB
通讯作者：
ROBINSON, GB
影响因子：
3
作者：
Chen, CH;Hansma, HG
通讯作者：
Hansma, HG