Immunocytochemical detection of milk proteins in tracheal aspirates of ventilated infants: A pilot study
Immunocytochemical detection of milk proteins in tracheal aspirates of ventilated infants: A pilot study
复制标题
通气婴儿气管抽吸物中乳蛋白的免疫细胞化学检测：一项初步研究
作者：
Jamey Miller;G. Colasurdo;A. Khan;Charu Jajoo;Tarak J. Patel;L. Fan;O. Elidemir
在本研究中，我们评价了机械通气婴儿气管吸出物中乳蛋白（α-乳白蛋白和β-乳球蛋白）的免疫细胞化学染色，并评估了这种染色技术是否支持接受口胃喂养婴儿的误吸临床诊断。本研究的潜在受试者为大型三级保健医院新生儿重症监护室中需要机械通气的所有新生儿。在需要机械通气的新生儿中，在引入肠内喂养之前和之后的不同时间点获得气管抽吸物。获得细胞并进行处理，用于α-乳白蛋白和β-乳球蛋白的免疫细胞化学染色。
In this study, we evaluated immunocytochemical staining for milk proteins (α‐lactalbumin and β‐lactoglobulin) in tracheal aspirates of mechanically ventilated infants, and assessed whether this staining technique supported a clinical diagnosis of aspiration in infants receiving orogastric feedings. All newborns requiring mechanical ventilation in the neonatal intensive care unit of a major tertiary care hospital were potential subjects for this study. Tracheal aspirates were obtained prior to the introduction of enteral feeding and at various time points thereafter in newborns requiring mechanical ventilation. Cells were obtained and processed for immunocytochemical staining of α‐lactalbumin and β‐lactoglobulin.
正常和过敏原致敏兔子中非肾上腺素能非胆碱能抑制系统的成熟。
DOI：
10.1152/ajplung.1994.267.6.l739
发表时间：
1994
期刊：
The American journal of physiology
影响因子：
--
作者：
Colasurdo,GN;Loader,JE;Graves,JP;Larsen,GL
通讯作者：
Larsen,GL
肺脂肪栓塞：镰状细胞性贫血中严重急性胸部综合征的明显原因。
影响因子：
20.3
作者：
Vichinsky,E;Williams,R;Das,M;Earles,AN;Lewis,N;Adler,A;McQuitty,J
通讯作者：
McQuitty,J