Daily running enhances molecular and physiological circadian rhythms in skeletal muscle.
Daily running enhances molecular and physiological circadian rhythms in skeletal muscle.
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日常跑步增强骨骼肌的分子和生理昼夜节律。
DOI：
10.1016/j.molmet.2022.101504
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发表时间：
2022-07
影响因子：
8.1
通讯作者：
Lamia, Katja A.
中科院分区：
文献类型：
--
作者：
Casanova-Vallve, Nuria;Duglan, Drew;Vaughan, Megan E.;Pariollaud, Marie;Handzlik, Michal K.;Fan, Weiwei;Yu, Ruth T.;Liddle, Christopher;Downes, Michael;Delezie, Julien;Mello, Rebecca;Chan, Alanna B.;Westermark, Pal O.;Metallo, Christian M.;Evans, Ronald M.;Lamia, Katja A.
运动是健康生活方式的重要组成部分，也是预防和管理代谢性疾病的关键策略。确定适应慢性体力活动的分子机制是代谢生理学的关键。昼夜节律广泛地调节新陈代谢，包括肌肉底物利用和运动能力。在这里，我们定义的分子和生理变化引起的日常周期自愿低强度的日常锻炼。将野生型C57 BL 6/J雄性和雌性小鼠在有或没有转轮的情况下圈养六周。在四个不同的时钟时间（ZT，开灯后的小时），使用电刺激或手动刺激，以激励在电动跑步机上持续跑步，测量最大跑步速度。从跖肌中分离的RNA在六个ZT进行测序，以建立日常活动对全基因组转录的影响。使用基因集富集分析（GSEA）和差异节律性检测（DODR）分析基因表达模式。运动前后分别测量血糖、乳酸、酮体、肌糖原和肝糖原。我们证明，使用温和的电击激励运行的负面影响小鼠的最大运行速度，并描述了一个手动的方法来激励啮齿动物运动研究中运行。使用这种方法，我们表明，一天中的时间会影响六周自愿轮跑所提供的运动能力的增加：当在小鼠夜间活动期开始时测量最大跑步速度时，没有可测量的益处来自每日自愿跑步的历史，而最大的性能增加发生在晚上结束时。我们发现，每天自愿运动显着重塑小鼠肌肉昼夜节律转录组。最后，我们描述了每天的节奏，在碳水化合物代谢与时间依赖性的反应，以适度的日常运动小鼠。总的来说，这些数据表明，慢性夜间体力活动显着重塑小鼠肌肉基因表达的每日节律，这反过来又支持运动表现的每日波动。每日自主跑步显著重塑小鼠肌肉昼夜节律转录组。每天自主跑最大限度地提高活跃期后期小鼠的跑速。肌肉和肝糖原含量在实验室小鼠中表现出强有力的每日节律。使用轻度电击刺激小鼠跑步会损害最大跑步速度。
Exercise is a critical component of a healthy lifestyle and a key strategy for the prevention and management of metabolic disease. Identifying molecular mechanisms underlying adaptation in response to chronic physical activity is of critical interest in metabolic physiology. Circadian rhythms broadly modulate metabolism, including muscle substrate utilization and exercise capacity. Here, we define the molecular and physiological changes induced across the daily cycle by voluntary low intensity daily exercise. Wildtype C57BL6/J male and female mice were housed with or without access to a running wheel for six weeks. Maximum running speed was measured at four different zeitgeber times (ZTs, hours after lights on) using either electrical or manual stimulation to motivate continued running on a motorized treadmill. RNA isolated from plantaris muscles at six ZTs was sequenced to establish the impact of daily activity on genome-wide transcription. Patterns of gene expression were analyzed using Gene Set Enrichment Analysis (GSEA) and Detection of Differential Rhythmicity (DODR). Blood glucose, lactate, and ketones, and muscle and liver glycogen were measured before and after exercise. We demonstrate that the use of mild electrical shocks to motivate running negatively impacts maximum running speed in mice, and describe a manual method to motivate running in rodent exercise studies. Using this method, we show that time of day influences the increase in exercise capacity afforded by six weeks of voluntary wheel running: when maximum running speed is measured at the beginning of the nighttime active period in mice, there is no measurable benefit from a history of daily voluntary running, while maximum increase in performance occurs at the end of the night. We show that daily voluntary exercise dramatically remodels the murine muscle circadian transcriptome. Finally, we describe daily rhythms in carbohydrate metabolism associated with the time-dependent response to moderate daily exercise in mice. Collectively, these data indicate that chronic nighttime physical activity dramatically remodels daily rhythms of murine muscle gene expression, which in turn support daily fluctuations in exercise performance. Daily voluntary running dramatically remodels the mouse muscle circadian transcriptome. Daily voluntary running maximally increases mouse running speed in the late active period. Muscle and liver glycogen content exhibit robust daily rhythms in laboratory mice. Use of mild electric shocks to motivate running in mice impairs maximum running speed.
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影响因子：
14.9
作者：
Gene Ontology Consortium
通讯作者：
Gene Ontology Consortium
影响因子：
8.1
作者：
Dyar KA;Ciciliot S;Wright LE;Biensø RS;Tagliazucchi GM;Patel VR;Forcato M;Paz MI;Gudiksen A;Solagna F;Albiero M;Moretti I;Eckel-Mahan KL;Baldi P;Sassone-Corsi P;Rizzuto R;Bicciato S;Pilegaard H;Blaauw B;Schiaffino S
通讯作者：
Schiaffino S
昼夜主义代谢的地图集揭示了时钟之间全系统的协调和通信。
影响因子：
64.5
作者：
Dyar KA;Lutter D;Artati A;Ceglia NJ;Liu Y;Armenta D;Jastroch M;Schneider S;de Mateo S;Cervantes M;Abbondante S;Tognini P;Orozco-Solis R;Kinouchi K;Wang C;Swerdloff R;Nadeef S;Masri S;Magistretti P;Orlando V;Borrelli E;Uhlenhaut NH;Baldi P;Adamski J;Tschöp MH;Eckel-Mahan K;Sassone-Corsi P
通讯作者：
Sassone-Corsi P
影响因子：
29
作者：
Fan W;Waizenegger W;Lin CS;Sorrentino V;He MX;Wall CE;Li H;Liddle C;Yu RT;Atkins AR;Auwerx J;Downes M;Evans RM
通讯作者：
Evans RM
影响因子：
5.8
作者：
Goodpaster, BH;He, J;Kelley, DE
通讯作者：
Kelley, DE