Visible Light Optical Coherence Tomography (OCT) Quantifies Subcellular Contributions to Outer Retinal Band 4.
Visible Light Optical Coherence Tomography (OCT) Quantifies Subcellular Contributions to Outer Retinal Band 4.
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可见光光学相干断层扫描(OCT)量化了亚细胞对视网膜外带4的贡献。
DOI：
10.1167/tvst.10.3.30
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发表时间：
2021-03-01
影响因子：
3
通讯作者：
Srinivasan VJ
中科院分区：
文献类型：
--
作者：
Zhang T;Kho AM;Yiu G;Srinivasan VJ
应用可见光光学相干断层扫描(OCT)研究亚细胞反射率对当前临床近红外(NIR)OCT显示的视网膜高反射带最外层(第4层)的贡献。对19例28眼(21~57岁)无眼病史的受试者进行了轴向分辨率为1.0µm的可见光OCT检查。所有眼均在4波段内一致显示出2个黄斑中心凹高反射区和3个黄斑中心凹外高反射区。NIR OCT偶见最外层的两条高反射带，推测为视网膜色素上皮(RPE)和Bruch膜(BM)。视网膜色素上皮厚度、基底膜厚度和视网膜色素上皮内反射率在黄斑上进行了地形图定量。发现一种修正RPE多次散射尾巴的方法既能提高BM强度分布的高斯拟合度，又能降低体内BM厚度的变异系数。黄斑BM厚度没有明显的地形差异。RPE厚度随偏心率的增加而减小。视网膜色素上皮内的可见光OCT信号强度向顶端加重，中心凹内的可见光OCT信号沿视网膜色素上皮层的衰减程度大于周边。推测的RPE和BM带的形态计量学与已知的解剖学相一致。RPE反射率向顶端的加权表明，黑素小体是年轻眼睛RPE后向散射和信号衰减的主要贡献者。通过实现RPE和BM的形态测量分析，可见光OCT破译了商业OCT系统通常显示的外视网膜带4的主要反射率贡献。
To use visible light optical coherence tomography (OCT) to investigate subcellular reflectivity contributions to the outermost (4th) of the retinal hyperreflective bands visualized by current clinical near-infrared (NIR) OCT. Visible light OCT, with 1.0 µm axial resolution, was performed in 28 eyes of 19 human subjects (21–57 years old) without history of ocular pathology. Two foveal and three extrafoveal hyperreflective zones were consistently depicted within band 4 in all eyes. The two outermost hyperreflective bands, occasionally visualized by NIR OCT, were presumed to be the retinal pigment epithelium (RPE) and Bruch's membrane (BM). RPE thickness, BM thickness, and RPE interior reflectivity were quantified topographically across the macula. A method for correcting RPE multiple scattering tails was found to both improve the Gaussian goodness-of-fit for the BM intensity profile and reduce the coefficient of variation of BM thickness in vivo. No major topographical differences in macular BM thickness were noted. RPE thickness decreased with increasing eccentricity. Visible light OCT signal intensity in the RPE was weighted to the apical side and attenuated more across the RPE in the fovea than peripherally. Morphometry of the presumed RPE and BM bands is consistent with known anatomy. Weighting of RPE reflectivity toward the apical side suggests that melanosomes are the predominant contributors to RPE backscattering and signal attenuation in young eyes. By enabling morphometric analysis of the RPE and BM, visible light OCT deciphers the main reflectivity contributions to outer retinal band 4, commonly visualized by commercial OCT systems.
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