Functional implications of the influence of ABCA1 on lipid microenvironment at the plasma membrane: a biophysical study
Functional implications of the influence of ABCA1 on lipid microenvironment at the plasma membrane: a biophysical study
复制标题
ABCA1 对质膜脂质微环境影响的功能意义：生物物理学研究
DOI：
10.1096/fj.08-122192
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Chimini G
中科院分区：
文献类型：
--
作者：
Zarubica A;Plazzo AP;Stöckl M;Trombik T;Hamon Y;Müller P;Pomorski T;Herrmann A;Chimini G
ABCA1 转运蛋白通过促进胆固醇向血浆受体的释放来协调细胞脂质稳态。然而，其分子机制尚不清楚。我们在此报告对活 HeLa 细胞质膜 ABCA1 脂质微环境的生物物理分析。通过荧光寿命成像显微镜（FLIM）监测插入膜的荧光脂质类似物的寿命，或分别监测阳离子传感器的膜易位，评估ABCA1诱导的膜属性的改变在外叶和内叶。对这些细胞囊泡质膜泡中专用探针的分配进行分析，可以看到 ABCA1 分配到液体无序相中，并证实了 ABCA1 破坏膜上脂质排列的稳定性的观点。通过与表达非活性转运蛋白的细胞进行比较来证明特异性。这些修饰的生理相关性最终通过在活性 ABCA1 的影响下膜迁移率和转铁蛋白受体功能的降低得到证明。总的来说，这些数据评估了控制膜上的横向和横向脂质分布是 ABCA1 的主要功能，并将胆固醇从细胞膜流出下游定位到甾醇重新分布到易于提取的膜池中。-Zarubica, A.、Plazzo, A.P.、Stockl, M.、Trombik, T.、Hamon, Y.、Muller, P., Pomorski, T., Herrmann, A., Chimini, G. ABCA1 对质膜脂质微环境影响的功能意义：生物物理学研究。FASEB J.23, 1775–1785 (2009)
The ABCA1 transporter orchestrates cellular lipid homeostasis by promoting the release of cholesterol to plasmatic acceptors. The molecular mechanism is, however, unknown. We report here on the biophysical analysis in living HeLa cells of the ABCA1 lipid microenvironment at the plasma membrane. The modifications of membrane attributes induced by ABCA1 were assessed at both the outer and inner leaflet by monitoring either the lifetime of membrane inserted fluorescent lipid analogues by fluorescence lifetime imaging microscopy (FLIM) or, respectively, the membrane translocation of cationic sensors. Analysis of the partitioning of dedicated probes in plasma membrane blebs vesiculated from these cells allowed visualization of ABCA1 partitioning into the liquid disordered‐like phase and corroborated the idea that ABCA1 destabilizes the lipid arrangement at the membrane. Specificity was demonstrated by comparison with cells expressing an inactive transporter. The physiological relevance of these modifications was finally demonstrated by the reduced membrane mobility and function of transferrin receptors under the influence of an active ABCA1. Collectively, these data assess that the control of both transversal and lateral lipid distribution at the membrane is the primary function of ABCA1 and positions the effluxes of cholesterol from cell membranes downstream to the redistribution of the sterol into readily extractable membrane pools.—Zarubica, A., Plazzo, A.P., Stockl, M., Trombik, T., Hamon, Y., Muller, P., Pomorski, T., Herrmann, A., Chimini, G. Functional implications of the influence of ABCA1 on lipid microenvironment at the plasma membrane: a biophysical study.FASEB J.23, 1775–1785 (2009)
登录
查看更多内容
ABCA1缺陷型细胞中的经贝贝磷脂运动。
影响因子：
3.7
作者：
Williamson, Patrick;Halleck, Margaret S.;Malowitz, Jonathan;Ng, Susan;Fan, Xiaoxuan;Krahling, Stephen;Remaley, Alan T.;Schlegel, Robert A.
通讯作者：
Schlegel, Robert A.
ABCA1 介导膜囊泡对 25-羟基胆固醇的高亲和力摄取以及完整细胞对氧甾醇的快速流出。
DOI：
10.1152/ajpcell.00055.2006
发表时间：
2006
期刊：
American journal of physiology. Cell physiology
影响因子：
--
作者：
S. Tam;Leo Mok;G. Chimini;M. Vasa;R. Deeley
通讯作者：
R. Deeley
影响因子：
30.8
作者：
S. Young;C. Fielding
通讯作者：
S. Young;C. Fielding
影响因子：
2.9
作者：
Y. Lange;Jin Ye;T. Steck
通讯作者：
Y. Lange;Jin Ye;T. Steck
影响因子：
3.4
作者：
Sengupta, Prabuddha;Hammond, Adam;Baird, Barbara
通讯作者：
Baird, Barbara