Quantitative analysis of calcium spikes in noisy fluorescent background.
Quantitative analysis of calcium spikes in noisy fluorescent background.
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DOI：
10.1371/journal.pone.0064394
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zahradník I
中科院分区：
文献类型：
--
作者：
Janicek R;Hotka M;Zahradníková A Jr;Zahradníková A;Zahradník I
用激光扫描共聚焦荧光显微镜研究细胞内钙信号。由于低信噪比，所需的时空分辨率使得钙信号的描述变得困难。在模型拟合的基础上，设计了一种新的钙峰分析方法。在随机变化的峰值参数和恒定振幅的高斯噪声合成数据集和恒定峰值参数和不同振幅的高斯噪声合成数据集上测试钙峰值描述的准确性和精密度。采用统计分析的方法对钉刺拟合算法的性能进行了评价。该程序被优化为可靠的估计钙峰值参数和解雇假事件。提出了一种校正线扫描图像中由于沿空间坐标顺序采集单个像素而导致的像素采集时间误差的算法。在Matlab中开发了新的软件，并提供了通用。它允许从x-t图像中对钙峰值的时间剖面进行交互式解剖，它们与预定义的函数相拟合，并在统计基础上接受结果，从而允许对心肌细胞中的钙信号进行有效的分析和可靠的描述，直到ryanodine受体的原位功能。
Intracellular calcium signals are studied by laser-scanning confocal fluorescence microscopy. The required spatio-temporal resolution makes description of calcium signals difficult because of the low signal-to-noise ratio. We designed a new procedure of calcium spike analysis based on their fitting with a model. The accuracy and precision of calcium spike description were tested on synthetic datasets generated either with randomly varied spike parameters and Gaussian noise of constant amplitude, or with constant spike parameters and Gaussian noise of various amplitudes. Statistical analysis was used to evaluate the performance of spike fitting algorithms. The procedure was optimized for reliable estimation of calcium spike parameters and for dismissal of false events. A new algorithm was introduced that corrects the acquisition time of pixels in line-scan images that is in error due to sequential acquisition of individual pixels along the space coordinate. New software was developed in Matlab and provided for general use. It allows interactive dissection of temporal profiles of calcium spikes from x-t images, their fitting with predefined function(s) and acceptance of results on statistical grounds, thus allowing efficient analysis and reliable description of calcium signaling in cardiac myocytes down to the in situ function of ryanodine receptors.
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